Exogenous expression of proteins in neurons using the biolistic particle delivery system.

Nikita Gamper; Mark S. Shapiro

doi:10.1385/1-59745-095-2:27

Exogenous expression of proteins in neurons using the biolistic particle delivery system.

Nikita Gamper, Mark S. Shapiro

Research output: Contribution to journal › Article

14 Scopus citations

Abstract

Exogenous expression of genes in mammalian neurons represents a substantial experimental challenge because of the low efficiency of commercially available liposomal transfection reagents for nondividing cells and considerable toxicity of viral transfection systems. In this chapter, we discuss application of the "biolistic" particle delivery system for heterologous expression of genes in primary neuron cultures. The method is based on the direct introduction of cDNA of interest into the nucleus by penetration with DNA-coated gold particles. With this approach, cDNA expression is independent of cell cycling and proliferation and is similar to intranuclear microinjection, with both avoiding cDNA delivery through the cytosol. Examples of successful transfection using PDS of rat superior cervical ganglion and trigeminal ganglion neurons are discussed.

Original language	English (US)
Pages (from-to)	27-38
Number of pages	12
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	337
DOIs	https://doi.org/10.1385/1-59745-095-2:27
State	Published - 2006
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics

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Gamper, N., & Shapiro, M. S. (2006). Exogenous expression of proteins in neurons using the biolistic particle delivery system. Methods in molecular biology (Clifton, N.J.), 337, 27-38. https://doi.org/10.1385/1-59745-095-2:27

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