Exogenous expression of proteins in neurons using the biolistic particle delivery system.

Nikita Gamper; Mark S. Shapiro

doi:10.1385/1-59745-095-2:27

Exogenous expression of proteins in neurons using the biolistic particle delivery system.

Nikita Gamper, Mark S. Shapiro

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Exogenous expression of genes in mammalian neurons represents a substantial experimental challenge because of the low efficiency of commercially available liposomal transfection reagents for nondividing cells and considerable toxicity of viral transfection systems. In this chapter, we discuss application of the "biolistic" particle delivery system for heterologous expression of genes in primary neuron cultures. The method is based on the direct introduction of cDNA of interest into the nucleus by penetration with DNA-coated gold particles. With this approach, cDNA expression is independent of cell cycling and proliferation and is similar to intranuclear microinjection, with both avoiding cDNA delivery through the cytosol. Examples of successful transfection using PDS of rat superior cervical ganglion and trigeminal ganglion neurons are discussed.

Original language	English (US)
Pages (from-to)	27-38
Number of pages	12
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	337
DOIs	https://doi.org/10.1385/1-59745-095-2:27
State	Published - 2006
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1385/1-59745-095-2:27

Fingerprint Dive into the research topics of 'Exogenous expression of proteins in neurons using the biolistic particle delivery system.'. Together they form a unique fingerprint.

Cite this

@article{22c49c978d5b4a549a28329030eaa1e4,

title = "Exogenous expression of proteins in neurons using the biolistic particle delivery system.",

abstract = "Exogenous expression of genes in mammalian neurons represents a substantial experimental challenge because of the low efficiency of commercially available liposomal transfection reagents for nondividing cells and considerable toxicity of viral transfection systems. In this chapter, we discuss application of the {"}biolistic{"} particle delivery system for heterologous expression of genes in primary neuron cultures. The method is based on the direct introduction of cDNA of interest into the nucleus by penetration with DNA-coated gold particles. With this approach, cDNA expression is independent of cell cycling and proliferation and is similar to intranuclear microinjection, with both avoiding cDNA delivery through the cytosol. Examples of successful transfection using PDS of rat superior cervical ganglion and trigeminal ganglion neurons are discussed.",

author = "Nikita Gamper and Shapiro, {Mark S.}",

note = "Copyright: This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine",

year = "2006",

doi = "10.1385/1-59745-095-2:27",

language = "English (US)",

volume = "337",

pages = "27--38",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Exogenous expression of proteins in neurons using the biolistic particle delivery system.

AU - Gamper, Nikita

AU - Shapiro, Mark S.

N1 - Copyright: This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine

PY - 2006

Y1 - 2006

N2 - Exogenous expression of genes in mammalian neurons represents a substantial experimental challenge because of the low efficiency of commercially available liposomal transfection reagents for nondividing cells and considerable toxicity of viral transfection systems. In this chapter, we discuss application of the "biolistic" particle delivery system for heterologous expression of genes in primary neuron cultures. The method is based on the direct introduction of cDNA of interest into the nucleus by penetration with DNA-coated gold particles. With this approach, cDNA expression is independent of cell cycling and proliferation and is similar to intranuclear microinjection, with both avoiding cDNA delivery through the cytosol. Examples of successful transfection using PDS of rat superior cervical ganglion and trigeminal ganglion neurons are discussed.

AB - Exogenous expression of genes in mammalian neurons represents a substantial experimental challenge because of the low efficiency of commercially available liposomal transfection reagents for nondividing cells and considerable toxicity of viral transfection systems. In this chapter, we discuss application of the "biolistic" particle delivery system for heterologous expression of genes in primary neuron cultures. The method is based on the direct introduction of cDNA of interest into the nucleus by penetration with DNA-coated gold particles. With this approach, cDNA expression is independent of cell cycling and proliferation and is similar to intranuclear microinjection, with both avoiding cDNA delivery through the cytosol. Examples of successful transfection using PDS of rat superior cervical ganglion and trigeminal ganglion neurons are discussed.

UR - http://www.scopus.com/inward/record.url?scp=34249913061&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249913061&partnerID=8YFLogxK

U2 - 10.1385/1-59745-095-2:27

DO - 10.1385/1-59745-095-2:27

M3 - Article

C2 - 16929936

AN - SCOPUS:34249913061

VL - 337

SP - 27

EP - 38

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

SN - 1064-3745

ER -

Exogenous expression of proteins in neurons using the biolistic particle delivery system.

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this