TY - JOUR
T1 - Exogenous expression of proteins in neurons using the biolistic particle delivery system.
AU - Gamper, Nikita
AU - Shapiro, Mark S.
PY - 2006
Y1 - 2006
N2 - Exogenous expression of genes in mammalian neurons represents a substantial experimental challenge because of the low efficiency of commercially available liposomal transfection reagents for nondividing cells and considerable toxicity of viral transfection systems. In this chapter, we discuss application of the "biolistic" particle delivery system for heterologous expression of genes in primary neuron cultures. The method is based on the direct introduction of cDNA of interest into the nucleus by penetration with DNA-coated gold particles. With this approach, cDNA expression is independent of cell cycling and proliferation and is similar to intranuclear microinjection, with both avoiding cDNA delivery through the cytosol. Examples of successful transfection using PDS of rat superior cervical ganglion and trigeminal ganglion neurons are discussed.
AB - Exogenous expression of genes in mammalian neurons represents a substantial experimental challenge because of the low efficiency of commercially available liposomal transfection reagents for nondividing cells and considerable toxicity of viral transfection systems. In this chapter, we discuss application of the "biolistic" particle delivery system for heterologous expression of genes in primary neuron cultures. The method is based on the direct introduction of cDNA of interest into the nucleus by penetration with DNA-coated gold particles. With this approach, cDNA expression is independent of cell cycling and proliferation and is similar to intranuclear microinjection, with both avoiding cDNA delivery through the cytosol. Examples of successful transfection using PDS of rat superior cervical ganglion and trigeminal ganglion neurons are discussed.
UR - http://www.scopus.com/inward/record.url?scp=34249913061&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34249913061&partnerID=8YFLogxK
U2 - 10.1385/1-59745-095-2:27
DO - 10.1385/1-59745-095-2:27
M3 - Article
C2 - 16929936
AN - SCOPUS:34249913061
VL - 337
SP - 27
EP - 38
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -