Exclusive expression of G-CSF receptor on myeloid progenitors in bone marrow CD34+ cells

Yasuhiro Ebihara, Ming Jiang Xu, Atsushi Manabe, Akira Kikuchi, Ryuhei Tanaka, Shunichi Kato, Tatsutoshi Nakahata, Kohichiro Tsuji

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Although granulocyte colony-stimulating factor (G-CSF) has been reported to act on cells of neutrophilic lineage, the administration of G-CSF to induce the mobilization of various haematopoietic progenitors into the circulation. We analysed the expression of receptors for G-CSF (G-CSFR) on human bone marrow and G-CSF-mobilized peripheral blood CD34+ cells, and examined the proliferation and differentiation capabilities of sorted CD34+G-CSFR+ and CD34+G-CSFR- cells using methylcellulose clonal culture. Flow cytometric analysis showed that G-CSFR was expressed on 14.9 ± 4.9% of bone marrow CD34+ cells, most of which were included in CD34+CD33+ and CD34+CD38+ cell fractions. In clonal cultures, CD34+G-CSFR+ cells produced only myeloid colonies, whereas CD34+G-CSFR- cells produced erythroid bursts, megakaryocyte and multilineage colonies. When incubated with the cytokine cocktail for 5 d, CD34+G-CSFR- cells generated CD34+G- CSFR+ myeloid progenitors. In G-CSF-mobilized peripheral blood, CD34+ cells contained 10.8 ± 5.8% of G-CSFR+ cells, most of which were also myeloid progenitors, although CD34+G-CSFR- cells contained a substantial number of myeloid progenitors. These results indicated that the expression of G-CSFR on CD34+ cells is restricted to myeloid progenitors, suggesting that the specific activity of G-CSF on myelopoiesis depends on the exclusive expression of its receptor on myeloid progenitors, and that the mobilization of various haematopoietic progenitors is not a direct effect of G-CSF in humans.

Original languageEnglish (US)
Pages (from-to)153-161
Number of pages9
JournalBritish Journal of Haematology
Volume109
Issue number1
DOIs
StatePublished - 2000

Keywords

  • Bone marrow
  • CD34
  • G-CSF receptor
  • G-CSF-mobilized peripheral blood
  • Myeloid progenitor

ASJC Scopus subject areas

  • Hematology

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