Examination of the brain mitochondrial lipidome using shotgun lipidomics

Michael A. Kiebish, Xianlin Han, Thomas N. Seyfried

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Contamination from subcellular organelles and myelin has hindered attempts to characterize the lipidome of brain mitochondria. A high degree of mitochondrial purity is required for accurate measurements of the content and molecular species composition of mitochondrial lipids. We devised a discontinuous Ficoll and sucrose gradient procedure for the isolation and purification of brain mitochondria free from any detectable contamination. Shotgun lipidomics was used to analyze the lipid composition of the brain mitochondria. These procedures can be used to determine whether intrinsic lipid abnormalities underlie mitochondrial dysfunction associated with neurological and neurodegenerative diseases.

Original languageEnglish (US)
Pages (from-to)3-18
Number of pages16
JournalMethods in Molecular Biology
StatePublished - 2009
Externally publishedYes


  • Brain
  • Lipidome
  • Mitochondria
  • Nonsynaptic
  • Synaptic

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


Dive into the research topics of 'Examination of the brain mitochondrial lipidome using shotgun lipidomics'. Together they form a unique fingerprint.

Cite this