Examination of the brain mitochondrial lipidome using shotgun lipidomics

Michael A. Kiebish, Xianlin Han, Thomas N. Seyfried

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Contamination from subcellular organelles and myelin has hindered attempts to characterize the lipidome of brain mitochondria. A high degree of mitochondrial purity is required for accurate measurements of the content and molecular species composition of mitochondrial lipids. We devised a discontinuous Ficoll and sucrose gradient procedure for the isolation and purification of brain mitochondria free from any detectable contamination. Shotgun lipidomics was used to analyze the lipid composition of the brain mitochondria. These procedures can be used to determine whether intrinsic lipid abnormalities underlie mitochondrial dysfunction associated with neurological and neurodegenerative diseases.

Original languageEnglish (US)
Pages (from-to)3-18
Number of pages16
JournalMethods in Molecular Biology
Volume579
DOIs
StatePublished - Jan 1 2009

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Keywords

  • Brain
  • Lipidome
  • Mitochondria
  • Nonsynaptic
  • Synaptic

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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