Ex vivo drug testing of patient-derived lung organoids to predict treatment responses for personalized medicine

Josephine A. Taverna, Chia Nung Hung, Madison Williams, Ryan Williams, Meizhen Chen, Samaneh Kamali, Vaishnavi Sambandam, Cheryl Hsiang-Ling Chiu, Pawel A. Osmulski, Maria E. Gaczynska, Daniel T. DeArmond, Christine Gaspard, Maria Mancini, Meena Kusi, Abhishek N. Pandya, Lina Song, Lingtao Jin, Paolo Schiavini, Chun Liang Chen

Research output: Contribution to journalReview articlepeer-review

1 Scopus citations


Lung cancer is the leading cause of global cancer-related mortality resulting in ∼ 1.8 million deaths annually. Systemic, molecular targeted, and immune therapies have provided significant improvements of survival outcomes for patients. However, drug resistance usually arises and there is an urgent need for novel therapy screening and personalized medicine. 3D patient-derived organoid (PDO) models have emerged as a more effective and efficient alternative for ex vivo drug screening than 2D cell culture and patient-derived xenograft (PDX) models. In this review, we performed an extensive search of lung cancer PDO-based ex vivo drug screening studies. Lung cancer PDOs were successfully established from fresh or bio-banked sections and/or biopsies, pleural effusions and PDX mouse models. PDOs were subject to ex vivo drug screening with chemotherapy, targeted therapy and/or immunotherapy. PDOs consistently recapitulated the genomic alterations and drug sensitivity of primary tumors. Although sample sizes of the previous studies were limited and some technical challenges remain, PDOs showed great promise in the screening of novel therapy drugs. With the technical advances of high throughput, tumor-on-chip, and combined microenvironment, the drug screening process using PDOs will enhance precision care of lung cancer patients.

Original languageEnglish (US)
Article number107533
JournalLung Cancer
StatePublished - Apr 2024


  • Clinical
  • Drug screening
  • High throughput
  • Lung cancer
  • Organoid
  • Personalized medicine
  • Pre-clinical
  • Translational
  • Tumor microenvironment

ASJC Scopus subject areas

  • Oncology
  • Pulmonary and Respiratory Medicine
  • Cancer Research


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