Evidence that multiple genetic variants of MC4R play a functional role in the regulation of energy expenditure and appetite in Hispanic children

Shelley A. Cole, Nancy F. Butte, V. Saroja Voruganti, Guowen Cai, Karin Haack, Jack W. Kent, John Blangero, Anthony G. Comuzzie, John D. McPherson, Richard A. Gibbs

Research output: Contribution to journalArticle

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Abstract

Background: Melanocortin-4-receptor (MC4R) haploinsufficiency is the most common form of monogenic obesity; however, the frequency of MC4R variants and their functional effects in general populations remain uncertain. Objective: The aim was to identify and characterize the effects of MC4R variants in Hispanic children. Design: MC4R was resequenced in 376 parents, and the identified single nucleotide polymorphisms (SNPs) were genotyped in 613 parents and 1016 children from the Viva la Familia cohort. Measured genotype analysis (MGA) tested associations between SNPs and phenotypes. Bayesian quantitative trait nucleotide (BQTN) analysis was used to infer the most likely functional polymorphisms influencing obesity-related traits. Results: Seven rare SNPs in coding and 18 SNPs in flanking regions of MC4R were identified. MGA showed suggestive associations between MC4R variants and body size, adiposity, glucose, insulin, leptin, ghrelin, energy expenditure, physical activity, and food intake. BQTN analysis identified SNP 1704 in a predicted micro-RNA target sequence in the downstream flanking region of MC4R as a strong, probable functional variant influencing total, sedentary, and moderate activities with posterior probabilities of 1.0. SNP 2132 was identified as a variant with a high probability (1.0) of exerting a functional effect on total energy expenditure and sleeping metabolic rate. SNP rs34114122 was selected as having likely functional effects on the appetite hormone ghrelin, with a posterior probability of 0.81. Conclusion: This comprehensive investigation provides strong evidence that MC4R genetic variants are likely to play a functional role in the regulation of weight, not only through energy intake but through energy expenditure.

Original languageEnglish (US)
Pages (from-to)191-199
Number of pages9
JournalAmerican Journal of Clinical Nutrition
Volume91
Issue number1
DOIs
StatePublished - Jan 1 2010
Externally publishedYes

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Receptor, Melanocortin, Type 4
Appetite
Hispanic Americans
Energy Metabolism
Single Nucleotide Polymorphism
Ghrelin
Nucleotides
Obesity
Parents
Genotype
Haploinsufficiency
Adiposity
Body Size
Leptin
Energy Intake
MicroRNAs
Eating
Hormones
Exercise
Insulin

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Medicine(all)

Cite this

Evidence that multiple genetic variants of MC4R play a functional role in the regulation of energy expenditure and appetite in Hispanic children. / Cole, Shelley A.; Butte, Nancy F.; Voruganti, V. Saroja; Cai, Guowen; Haack, Karin; Kent, Jack W.; Blangero, John; Comuzzie, Anthony G.; McPherson, John D.; Gibbs, Richard A.

In: American Journal of Clinical Nutrition, Vol. 91, No. 1, 01.01.2010, p. 191-199.

Research output: Contribution to journalArticle

Cole, SA, Butte, NF, Voruganti, VS, Cai, G, Haack, K, Kent, JW, Blangero, J, Comuzzie, AG, McPherson, JD & Gibbs, RA 2010, 'Evidence that multiple genetic variants of MC4R play a functional role in the regulation of energy expenditure and appetite in Hispanic children', American Journal of Clinical Nutrition, vol. 91, no. 1, pp. 191-199. https://doi.org/10.3945/ajcn.2009.28514
Cole, Shelley A. ; Butte, Nancy F. ; Voruganti, V. Saroja ; Cai, Guowen ; Haack, Karin ; Kent, Jack W. ; Blangero, John ; Comuzzie, Anthony G. ; McPherson, John D. ; Gibbs, Richard A. / Evidence that multiple genetic variants of MC4R play a functional role in the regulation of energy expenditure and appetite in Hispanic children. In: American Journal of Clinical Nutrition. 2010 ; Vol. 91, No. 1. pp. 191-199.
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abstract = "Background: Melanocortin-4-receptor (MC4R) haploinsufficiency is the most common form of monogenic obesity; however, the frequency of MC4R variants and their functional effects in general populations remain uncertain. Objective: The aim was to identify and characterize the effects of MC4R variants in Hispanic children. Design: MC4R was resequenced in 376 parents, and the identified single nucleotide polymorphisms (SNPs) were genotyped in 613 parents and 1016 children from the Viva la Familia cohort. Measured genotype analysis (MGA) tested associations between SNPs and phenotypes. Bayesian quantitative trait nucleotide (BQTN) analysis was used to infer the most likely functional polymorphisms influencing obesity-related traits. Results: Seven rare SNPs in coding and 18 SNPs in flanking regions of MC4R were identified. MGA showed suggestive associations between MC4R variants and body size, adiposity, glucose, insulin, leptin, ghrelin, energy expenditure, physical activity, and food intake. BQTN analysis identified SNP 1704 in a predicted micro-RNA target sequence in the downstream flanking region of MC4R as a strong, probable functional variant influencing total, sedentary, and moderate activities with posterior probabilities of 1.0. SNP 2132 was identified as a variant with a high probability (1.0) of exerting a functional effect on total energy expenditure and sleeping metabolic rate. SNP rs34114122 was selected as having likely functional effects on the appetite hormone ghrelin, with a posterior probability of 0.81. Conclusion: This comprehensive investigation provides strong evidence that MC4R genetic variants are likely to play a functional role in the regulation of weight, not only through energy intake but through energy expenditure.",
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AU - Butte, Nancy F.

AU - Voruganti, V. Saroja

AU - Cai, Guowen

AU - Haack, Karin

AU - Kent, Jack W.

AU - Blangero, John

AU - Comuzzie, Anthony G.

AU - McPherson, John D.

AU - Gibbs, Richard A.

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N2 - Background: Melanocortin-4-receptor (MC4R) haploinsufficiency is the most common form of monogenic obesity; however, the frequency of MC4R variants and their functional effects in general populations remain uncertain. Objective: The aim was to identify and characterize the effects of MC4R variants in Hispanic children. Design: MC4R was resequenced in 376 parents, and the identified single nucleotide polymorphisms (SNPs) were genotyped in 613 parents and 1016 children from the Viva la Familia cohort. Measured genotype analysis (MGA) tested associations between SNPs and phenotypes. Bayesian quantitative trait nucleotide (BQTN) analysis was used to infer the most likely functional polymorphisms influencing obesity-related traits. Results: Seven rare SNPs in coding and 18 SNPs in flanking regions of MC4R were identified. MGA showed suggestive associations between MC4R variants and body size, adiposity, glucose, insulin, leptin, ghrelin, energy expenditure, physical activity, and food intake. BQTN analysis identified SNP 1704 in a predicted micro-RNA target sequence in the downstream flanking region of MC4R as a strong, probable functional variant influencing total, sedentary, and moderate activities with posterior probabilities of 1.0. SNP 2132 was identified as a variant with a high probability (1.0) of exerting a functional effect on total energy expenditure and sleeping metabolic rate. SNP rs34114122 was selected as having likely functional effects on the appetite hormone ghrelin, with a posterior probability of 0.81. Conclusion: This comprehensive investigation provides strong evidence that MC4R genetic variants are likely to play a functional role in the regulation of weight, not only through energy intake but through energy expenditure.

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