Evidence that AVP receptors in AtT-20 corticotrophs are not coupled to secretion of POMC-derived peptides

Bernadette Lutz-Bucher, Lydie Jeandel, Seymour Heisler, James L. Roberts, Bernard Koch

Research output: Contribution to journalArticle

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Abstract

This study reports the presence in AtT-20 corticotrophs of high affinity-low capacity receptors for arginine-vasopressin (AVP), whose binding capacity was considerably enhanced by the divalent metal ion nickel. These binding sites, when analyzed in the presence of nickel, showed high affinity for AVP, vasotocin and oxytocin, but recognized to a lesser extent the V2-agonist 1-deamino-AVP, as well as V1-antagonists. Surprisingly, AVP failed to alter secretion of proopiomelanocortin (POMC)-derived peptides from the cells or corticotropin-releasing factor (CRF)-induced cAMP synthesis, as reported in normal corticotrophs. Exposure of cells to CRF elicited an increase in mRNAPOMC levels, while, in contrast, AVP was without significant effect. It thus appears that in AtT-20 tumor cells, the AVP receptors are not coupled to either the biochemical or biological cellular response.

Original languageEnglish (US)
Pages (from-to)161-167
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume53
Issue number3
DOIs
Publication statusPublished - Oct 1987

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Keywords

  • ACTH and α-MSH secretion
  • Arginine-vasopressin receptor
  • AtT-20 corticotroph
  • mRNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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