Evidence of porcine endogenous retroviruses in porcine factor VIII and evaluation of transmission to recipients with hemophilia

Walid Heneine, William M. Switzer, J. Michael Soucie, Bruce L. Evatt, Vedapuri Shanmugam, Guillermo Vazquez Rosales, Aprille Matthews, Paul Sandstrom, Thomas M. Folks

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    Abstract

    Since 1984, unheated porcine clotting factor VIII (Hyate:C) has been used to treat severe bleeding episodes in persons with hemophilia who have antibodies to human clotting factor. We document the presence of porcine endogenous retrovirus (PERV) in plasma samples of pigs and in clinical lots of Hyate:C. Both gag and pol PERV RNA sequences were detected by reverse-transcriptase (RT) polymerase chain reaction in 13 of 13 lots of Hyate:C tested. Among 10 of these lots, RT activity also was detected, which confirms the presence of retroviral particles. To assess the transmission of PERV to Hyate:C recipients, we tested serum specimens from 88 recipients of Hyate:C and 23 noninfused control subjects for anti-PERV antibodies by using a Western blot assay. None of the samples was positive. Our data document that PERV particles are a common contaminant of Hyate:C products and suggest that the risk of PERV transmission from these percutaneous exposures is very low.

    Original languageEnglish (US)
    Pages (from-to)648-652
    Number of pages5
    JournalJournal of Infectious Diseases
    Volume183
    Issue number4
    DOIs
    Publication statusPublished - Feb 15 2001

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    ASJC Scopus subject areas

    • Immunology and Allergy
    • Infectious Diseases

    Cite this

    Heneine, W., Switzer, W. M., Soucie, J. M., Evatt, B. L., Shanmugam, V., Rosales, G. V., ... Folks, T. M. (2001). Evidence of porcine endogenous retroviruses in porcine factor VIII and evaluation of transmission to recipients with hemophilia. Journal of Infectious Diseases, 183(4), 648-652. https://doi.org/10.1086/318540