Evidence of nef truncation in human immunodeficiency virus type 2 infection

William M. Switzer; Stefan Wiktor; Vincent Soriano; Antonio Silva-Graça; Kamal Mansinho; Issa Malik Coulibaly; Ehounou Ekpini; Alan E. Greenberg; Thomas M. Folks; Walid Heneine

doi:10.1086/513819

Evidence of nef truncation in human immunodeficiency virus type 2 infection

William M. Switzer, Stefan Wiktor, Vincent Soriano, Antonio Silva-Graça, Kamal Mansinho, Issa Malik Coulibaly, Ehounou Ekpini, Alan E. Greenberg, Thomas M. Folks, Walid Heneine

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Human immunodeficiency virus (HIV)-2 differs from HIV-1 in its relative lower transmissibility and pathogenicity. To understand the virologic basis of these differences, the nef gene from HIV-2-seropositive persons was analyzed because of its importance for disease progression in the genetically related simian immunodeficiency virus (SIV(MAC)). Proviral nef sequences from 60 HIV-2-infected persons were amplified from peripheral blood lymphocytes, and nef open-reading frames were screened by a transcription and translation assay for the presence of full-length (32- to 36-kDa) or truncated (<32 kDa) Nef proteins. Overall. 6 (10%) of 60 persons had truncated Nef proteins; of these, 5 were among the 36 asymptomatic subjects (13.9%) and only 1 was among the 24 symptomatic subjects (4.2%) (P =.23). The results of this study document the presence of defective nef genes in HIV-2 infections with a prevalence higher than that previously seen in HIV-1-infected cohorts of long-term nonprogressors or patients with AIDS.

Original language	English (US)
Pages (from-to)	65-71
Number of pages	7
Journal	Journal of Infectious Diseases
Volume	177
Issue number	1
DOIs	https://doi.org/10.1086/513819
State	Published - 1998

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

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Evidence of nef truncation in human immunodeficiency virus type 2 infection. / Switzer, William M.; Wiktor, Stefan; Soriano, Vincent; Silva-Graça, Antonio; Mansinho, Kamal; Coulibaly, Issa Malik; Ekpini, Ehounou; Greenberg, Alan E.; Folks, Thomas M.; Heneine, Walid.

In: Journal of Infectious Diseases, Vol. 177, No. 1, 1998, p. 65-71.

Research output: Contribution to journal › Article › peer-review

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title = "Evidence of nef truncation in human immunodeficiency virus type 2 infection",

abstract = "Human immunodeficiency virus (HIV)-2 differs from HIV-1 in its relative lower transmissibility and pathogenicity. To understand the virologic basis of these differences, the nef gene from HIV-2-seropositive persons was analyzed because of its importance for disease progression in the genetically related simian immunodeficiency virus (SIV(MAC)). Proviral nef sequences from 60 HIV-2-infected persons were amplified from peripheral blood lymphocytes, and nef open-reading frames were screened by a transcription and translation assay for the presence of full-length (32- to 36-kDa) or truncated (<32 kDa) Nef proteins. Overall. 6 (10%) of 60 persons had truncated Nef proteins; of these, 5 were among the 36 asymptomatic subjects (13.9%) and only 1 was among the 24 symptomatic subjects (4.2%) (P =.23). The results of this study document the presence of defective nef genes in HIV-2 infections with a prevalence higher than that previously seen in HIV-1-infected cohorts of long-term nonprogressors or patients with AIDS.",

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doi = "10.1086/513819",

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AU - Switzer, William M.

AU - Wiktor, Stefan

AU - Soriano, Vincent

AU - Silva-Graça, Antonio

AU - Mansinho, Kamal

AU - Coulibaly, Issa Malik

AU - Ekpini, Ehounou

AU - Greenberg, Alan E.

AU - Folks, Thomas M.

AU - Heneine, Walid

PY - 1998

Y1 - 1998

N2 - Human immunodeficiency virus (HIV)-2 differs from HIV-1 in its relative lower transmissibility and pathogenicity. To understand the virologic basis of these differences, the nef gene from HIV-2-seropositive persons was analyzed because of its importance for disease progression in the genetically related simian immunodeficiency virus (SIV(MAC)). Proviral nef sequences from 60 HIV-2-infected persons were amplified from peripheral blood lymphocytes, and nef open-reading frames were screened by a transcription and translation assay for the presence of full-length (32- to 36-kDa) or truncated (<32 kDa) Nef proteins. Overall. 6 (10%) of 60 persons had truncated Nef proteins; of these, 5 were among the 36 asymptomatic subjects (13.9%) and only 1 was among the 24 symptomatic subjects (4.2%) (P =.23). The results of this study document the presence of defective nef genes in HIV-2 infections with a prevalence higher than that previously seen in HIV-1-infected cohorts of long-term nonprogressors or patients with AIDS.

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Evidence of nef truncation in human immunodeficiency virus type 2 infection

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