Evidence of nef truncation in human immunodeficiency virus type 2 infection

William M. Switzer, Stefan Wiktor, Vincent Soriano, Antonio Silva-Graça, Kamal Mansinho, Issa Malik Coulibaly, Ehounou Ekpini, Alan E. Greenberg, Thomas M. Folks, Walid Heneine

Research output: Contribution to journalArticle

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Abstract

Human immunodeficiency virus (HIV)-2 differs from HIV-1 in its relative lower transmissibility and pathogenicity. To understand the virologic basis of these differences, the nef gene from HIV-2-seropositive persons was analyzed because of its importance for disease progression in the genetically related simian immunodeficiency virus (SIV(MAC)). Proviral nef sequences from 60 HIV-2-infected persons were amplified from peripheral blood lymphocytes, and nef open-reading frames were screened by a transcription and translation assay for the presence of full-length (32- to 36-kDa) or truncated (<32 kDa) Nef proteins. Overall. 6 (10%) of 60 persons had truncated Nef proteins; of these, 5 were among the 36 asymptomatic subjects (13.9%) and only 1 was among the 24 symptomatic subjects (4.2%) (P =.23). The results of this study document the presence of defective nef genes in HIV-2 infections with a prevalence higher than that previously seen in HIV-1-infected cohorts of long-term nonprogressors or patients with AIDS.

Original languageEnglish (US)
Pages (from-to)65-71
Number of pages7
JournalJournal of Infectious Diseases
Volume177
Issue number1
StatePublished - 1998
Externally publishedYes

Fingerprint

HIV-2
Virus Diseases
nef Genes
nef Gene Products
HIV-1
Simian Immunodeficiency Virus
Open Reading Frames
Virulence
Disease Progression
Acquired Immunodeficiency Syndrome
Lymphocytes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Switzer, W. M., Wiktor, S., Soriano, V., Silva-Graça, A., Mansinho, K., Coulibaly, I. M., ... Heneine, W. (1998). Evidence of nef truncation in human immunodeficiency virus type 2 infection. Journal of Infectious Diseases, 177(1), 65-71.

Evidence of nef truncation in human immunodeficiency virus type 2 infection. / Switzer, William M.; Wiktor, Stefan; Soriano, Vincent; Silva-Graça, Antonio; Mansinho, Kamal; Coulibaly, Issa Malik; Ekpini, Ehounou; Greenberg, Alan E.; Folks, Thomas M.; Heneine, Walid.

In: Journal of Infectious Diseases, Vol. 177, No. 1, 1998, p. 65-71.

Research output: Contribution to journalArticle

Switzer, WM, Wiktor, S, Soriano, V, Silva-Graça, A, Mansinho, K, Coulibaly, IM, Ekpini, E, Greenberg, AE, Folks, TM & Heneine, W 1998, 'Evidence of nef truncation in human immunodeficiency virus type 2 infection', Journal of Infectious Diseases, vol. 177, no. 1, pp. 65-71.
Switzer WM, Wiktor S, Soriano V, Silva-Graça A, Mansinho K, Coulibaly IM et al. Evidence of nef truncation in human immunodeficiency virus type 2 infection. Journal of Infectious Diseases. 1998;177(1):65-71.
Switzer, William M. ; Wiktor, Stefan ; Soriano, Vincent ; Silva-Graça, Antonio ; Mansinho, Kamal ; Coulibaly, Issa Malik ; Ekpini, Ehounou ; Greenberg, Alan E. ; Folks, Thomas M. ; Heneine, Walid. / Evidence of nef truncation in human immunodeficiency virus type 2 infection. In: Journal of Infectious Diseases. 1998 ; Vol. 177, No. 1. pp. 65-71.
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