Abstract
Human immunodeficiency virus (HIV)-2 differs from HIV-1 in its relative lower transmissibility and pathogenicity. To understand the virologic basis of these differences, the nef gene from HIV-2-seropositive persons was analyzed because of its importance for disease progression in the genetically related simian immunodeficiency virus (SIV(MAC)). Proviral nef sequences from 60 HIV-2-infected persons were amplified from peripheral blood lymphocytes, and nef open-reading frames were screened by a transcription and translation assay for the presence of full-length (32- to 36-kDa) or truncated (<32 kDa) Nef proteins. Overall. 6 (10%) of 60 persons had truncated Nef proteins; of these, 5 were among the 36 asymptomatic subjects (13.9%) and only 1 was among the 24 symptomatic subjects (4.2%) (P =.23). The results of this study document the presence of defective nef genes in HIV-2 infections with a prevalence higher than that previously seen in HIV-1-infected cohorts of long-term nonprogressors or patients with AIDS.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 65-71 |
| Number of pages | 7 |
| Journal | Journal of Infectious Diseases |
| Volume | 177 |
| Issue number | 1 |
| State | Published - 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health
- Immunology
Cite this
Evidence of nef truncation in human immunodeficiency virus type 2 infection. / Switzer, William M.; Wiktor, Stefan; Soriano, Vincent; Silva-Graça, Antonio; Mansinho, Kamal; Coulibaly, Issa Malik; Ekpini, Ehounou; Greenberg, Alan E.; Folks, Thomas M.; Heneine, Walid.
In: Journal of Infectious Diseases, Vol. 177, No. 1, 1998, p. 65-71.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evidence of nef truncation in human immunodeficiency virus type 2 infection
AU - Switzer, William M.
AU - Wiktor, Stefan
AU - Soriano, Vincent
AU - Silva-Graça, Antonio
AU - Mansinho, Kamal
AU - Coulibaly, Issa Malik
AU - Ekpini, Ehounou
AU - Greenberg, Alan E.
AU - Folks, Thomas M.
AU - Heneine, Walid
PY - 1998
Y1 - 1998
N2 - Human immunodeficiency virus (HIV)-2 differs from HIV-1 in its relative lower transmissibility and pathogenicity. To understand the virologic basis of these differences, the nef gene from HIV-2-seropositive persons was analyzed because of its importance for disease progression in the genetically related simian immunodeficiency virus (SIV(MAC)). Proviral nef sequences from 60 HIV-2-infected persons were amplified from peripheral blood lymphocytes, and nef open-reading frames were screened by a transcription and translation assay for the presence of full-length (32- to 36-kDa) or truncated (<32 kDa) Nef proteins. Overall. 6 (10%) of 60 persons had truncated Nef proteins; of these, 5 were among the 36 asymptomatic subjects (13.9%) and only 1 was among the 24 symptomatic subjects (4.2%) (P =.23). The results of this study document the presence of defective nef genes in HIV-2 infections with a prevalence higher than that previously seen in HIV-1-infected cohorts of long-term nonprogressors or patients with AIDS.
AB - Human immunodeficiency virus (HIV)-2 differs from HIV-1 in its relative lower transmissibility and pathogenicity. To understand the virologic basis of these differences, the nef gene from HIV-2-seropositive persons was analyzed because of its importance for disease progression in the genetically related simian immunodeficiency virus (SIV(MAC)). Proviral nef sequences from 60 HIV-2-infected persons were amplified from peripheral blood lymphocytes, and nef open-reading frames were screened by a transcription and translation assay for the presence of full-length (32- to 36-kDa) or truncated (<32 kDa) Nef proteins. Overall. 6 (10%) of 60 persons had truncated Nef proteins; of these, 5 were among the 36 asymptomatic subjects (13.9%) and only 1 was among the 24 symptomatic subjects (4.2%) (P =.23). The results of this study document the presence of defective nef genes in HIV-2 infections with a prevalence higher than that previously seen in HIV-1-infected cohorts of long-term nonprogressors or patients with AIDS.
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UR - http://www.scopus.com/inward/citedby.url?scp=17344373542&partnerID=8YFLogxK
M3 - Article
C2 - 9419171
AN - SCOPUS:17344373542
VL - 177
SP - 65
EP - 71
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 1
ER -