Evidence of nef truncation in human immunodeficiency virus type 2 infection

William M. Switzer; Stefan Wiktor; Vincent Soriano; Antonio Silva-Graça; Kamal Mansinho; Issa Malik Coulibaly; Ehounou Ekpini; Alan E. Greenberg; Thomas M. Folks; Walid Heneine

doi:10.1086/513819

Evidence of nef truncation in human immunodeficiency virus type 2 infection

William M. Switzer, Stefan Wiktor, Vincent Soriano, Antonio Silva-Graça, Kamal Mansinho, Issa Malik Coulibaly, Ehounou Ekpini, Alan E. Greenberg, Thomas M. Folks, Walid Heneine

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Human immunodeficiency virus (HIV)-2 differs from HIV-1 in its relative lower transmissibility and pathogenicity. To understand the virologic basis of these differences, the nef gene from HIV-2-seropositive persons was analyzed because of its importance for disease progression in the genetically related simian immunodeficiency virus (SIV(MAC)). Proviral nef sequences from 60 HIV-2-infected persons were amplified from peripheral blood lymphocytes, and nef open-reading frames were screened by a transcription and translation assay for the presence of full-length (32- to 36-kDa) or truncated (<32 kDa) Nef proteins. Overall. 6 (10%) of 60 persons had truncated Nef proteins; of these, 5 were among the 36 asymptomatic subjects (13.9%) and only 1 was among the 24 symptomatic subjects (4.2%) (P =.23). The results of this study document the presence of defective nef genes in HIV-2 infections with a prevalence higher than that previously seen in HIV-1-infected cohorts of long-term nonprogressors or patients with AIDS.

Original language	English (US)
Pages (from-to)	65-71
Number of pages	7
Journal	Journal of Infectious Diseases
Volume	177
Issue number	1
DOIs	https://doi.org/10.1086/513819
State	Published - 1998

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

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10.1086/513819

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Evidence of nef truncation in human immunodeficiency virus type 2 infection. / Switzer, William M.; Wiktor, Stefan; Soriano, Vincent; Silva-Graça, Antonio; Mansinho, Kamal; Coulibaly, Issa Malik; Ekpini, Ehounou; Greenberg, Alan E.; Folks, Thomas M.; Heneine, Walid.

In: Journal of Infectious Diseases, Vol. 177, No. 1, 1998, p. 65-71.

Research output: Contribution to journal › Article › peer-review

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title = "Evidence of nef truncation in human immunodeficiency virus type 2 infection",

abstract = "Human immunodeficiency virus (HIV)-2 differs from HIV-1 in its relative lower transmissibility and pathogenicity. To understand the virologic basis of these differences, the nef gene from HIV-2-seropositive persons was analyzed because of its importance for disease progression in the genetically related simian immunodeficiency virus (SIV(MAC)). Proviral nef sequences from 60 HIV-2-infected persons were amplified from peripheral blood lymphocytes, and nef open-reading frames were screened by a transcription and translation assay for the presence of full-length (32- to 36-kDa) or truncated (<32 kDa) Nef proteins. Overall. 6 (10%) of 60 persons had truncated Nef proteins; of these, 5 were among the 36 asymptomatic subjects (13.9%) and only 1 was among the 24 symptomatic subjects (4.2%) (P =.23). The results of this study document the presence of defective nef genes in HIV-2 infections with a prevalence higher than that previously seen in HIV-1-infected cohorts of long-term nonprogressors or patients with AIDS.",

author = "Switzer, {William M.} and Stefan Wiktor and Vincent Soriano and Antonio Silva-Gra{\c c}a and Kamal Mansinho and Coulibaly, {Issa Malik} and Ehounou Ekpini and Greenberg, {Alan E.} and Folks, {Thomas M.} and Walid Heneine",

note = "Funding Information: The HIV-2ST (pJSP4-27/H6) plasmid was provided by the National Institute of Health AIDS Research and Reference Reagent Program. We thank Danuta Pieniazek, Renu Lal, Tim Dondero, Dale Hu, and John Nekengasong for critical review of the manuscript.",

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AU - Switzer, William M.

AU - Wiktor, Stefan

AU - Soriano, Vincent

AU - Silva-Graça, Antonio

AU - Mansinho, Kamal

AU - Coulibaly, Issa Malik

AU - Ekpini, Ehounou

AU - Greenberg, Alan E.

AU - Folks, Thomas M.

AU - Heneine, Walid

N1 - Funding Information: The HIV-2ST (pJSP4-27/H6) plasmid was provided by the National Institute of Health AIDS Research and Reference Reagent Program. We thank Danuta Pieniazek, Renu Lal, Tim Dondero, Dale Hu, and John Nekengasong for critical review of the manuscript.

PY - 1998

Y1 - 1998

N2 - Human immunodeficiency virus (HIV)-2 differs from HIV-1 in its relative lower transmissibility and pathogenicity. To understand the virologic basis of these differences, the nef gene from HIV-2-seropositive persons was analyzed because of its importance for disease progression in the genetically related simian immunodeficiency virus (SIV(MAC)). Proviral nef sequences from 60 HIV-2-infected persons were amplified from peripheral blood lymphocytes, and nef open-reading frames were screened by a transcription and translation assay for the presence of full-length (32- to 36-kDa) or truncated (<32 kDa) Nef proteins. Overall. 6 (10%) of 60 persons had truncated Nef proteins; of these, 5 were among the 36 asymptomatic subjects (13.9%) and only 1 was among the 24 symptomatic subjects (4.2%) (P =.23). The results of this study document the presence of defective nef genes in HIV-2 infections with a prevalence higher than that previously seen in HIV-1-infected cohorts of long-term nonprogressors or patients with AIDS.

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