Evidence for RNA-RNA cross-link formation in Escherichia coli ribosomes

Christian Zwieb, Alex Ross, Jutta Rinke, Marlis Meinke, Richard Brimacombe

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Evidence is presented in three separate cases for the formation of RNA-RNA cross-links in intact E. coli ribosomes and ribosomal subunits. The first case is a cross-link between the 18S and 13S regions of the 23S RNA, induced by ultraviolet irradiation. The second is a cross-link at the subunit interface, generated by the bifunctional reagent bis-(2-chloroethyl)-amine. The third example is a cross-link between sections O'-D and P-A of the 16S RNA, induced as in the first case by ultraviolet irradiation. The RNA-RNA cross-links can be identified as such, despite the complications introduced by concomitant RNA-protein cross-linking reactions. The experiments represent a first attempt to introduce RNA-RNA cross-linking into studies of the topographical organization of the RNA within the ribosom.

Original languageEnglish (US)
Pages (from-to)2705-2720
Number of pages16
JournalNucleic acids research
Volume5
Issue number8
DOIs
StatePublished - Aug 1978

ASJC Scopus subject areas

  • Genetics

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