Evaluation of the necessity for rapid inactivation of brain enzymes prior to analysis of norepinephrine dopamine and serotonin in the mouse

Susan T. Weintraub; William B. Stavinoha; Robert L. Pike; William W. Morgan; Arvind T. Modak; Stephen H. Koslow; Le Roy Blank

doi:10.1016/0024-3205(75)90162-9

Evaluation of the necessity for rapid inactivation of brain enzymes prior to analysis of norepinephrine dopamine and serotonin in the mouse

Susan T. Weintraub, William B. Stavinoha, Robert L. Pike, William W. Morgan, Arvind T. Modak, Stephen H. Koslow, Le Roy Blank

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Norepinephrine, dopamine and serotonin concentrations were measured in mouse whole brain. Animals were killed either by decapitation or by exposure to 250 msec microwave irradiation which produces irreversible inactivation of brain enzymes. The biogenic amines were determined by mass fragmentometry, fluorometry and by a combination of high performance liquid chromatography and an electrochemical detector. No differences were found in the levels of these neurochemicals between the two methods of animal sacrifice. Therefore, rapid inactivation of brain enzymes is not necessary prior to analysis for catecholamines and serotonin in mouse whole brain.

Original language	English (US)
Pages (from-to)	1423-1427
Number of pages	5
Journal	Life Sciences
Volume	17
Issue number	9
DOIs	https://doi.org/10.1016/0024-3205(75)90162-9
State	Published - Nov 1 1975

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(75)90162-9

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title = "Evaluation of the necessity for rapid inactivation of brain enzymes prior to analysis of norepinephrine dopamine and serotonin in the mouse",

abstract = "Norepinephrine, dopamine and serotonin concentrations were measured in mouse whole brain. Animals were killed either by decapitation or by exposure to 250 msec microwave irradiation which produces irreversible inactivation of brain enzymes. The biogenic amines were determined by mass fragmentometry, fluorometry and by a combination of high performance liquid chromatography and an electrochemical detector. No differences were found in the levels of these neurochemicals between the two methods of animal sacrifice. Therefore, rapid inactivation of brain enzymes is not necessary prior to analysis for catecholamines and serotonin in mouse whole brain.",

author = "Weintraub, {Susan T.} and Stavinoha, {William B.} and Pike, {Robert L.} and Morgan, {William W.} and Modak, {Arvind T.} and Koslow, {Stephen H.} and Blank, {Le Roy}",

note = "Funding Information: This work was supported in part by NIMH grant number 1-ROI-MHZ5168-01 to WBS, NIH grant number 5-RO1-D00755-02 to WWM and Cottrell grant from Research Corporation to LB .",

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AU - Weintraub, Susan T.

AU - Stavinoha, William B.

AU - Pike, Robert L.

AU - Morgan, William W.

AU - Modak, Arvind T.

AU - Koslow, Stephen H.

AU - Blank, Le Roy

N1 - Funding Information: This work was supported in part by NIMH grant number 1-ROI-MHZ5168-01 to WBS, NIH grant number 5-RO1-D00755-02 to WWM and Cottrell grant from Research Corporation to LB .

PY - 1975/11/1

Y1 - 1975/11/1

N2 - Norepinephrine, dopamine and serotonin concentrations were measured in mouse whole brain. Animals were killed either by decapitation or by exposure to 250 msec microwave irradiation which produces irreversible inactivation of brain enzymes. The biogenic amines were determined by mass fragmentometry, fluorometry and by a combination of high performance liquid chromatography and an electrochemical detector. No differences were found in the levels of these neurochemicals between the two methods of animal sacrifice. Therefore, rapid inactivation of brain enzymes is not necessary prior to analysis for catecholamines and serotonin in mouse whole brain.

AB - Norepinephrine, dopamine and serotonin concentrations were measured in mouse whole brain. Animals were killed either by decapitation or by exposure to 250 msec microwave irradiation which produces irreversible inactivation of brain enzymes. The biogenic amines were determined by mass fragmentometry, fluorometry and by a combination of high performance liquid chromatography and an electrochemical detector. No differences were found in the levels of these neurochemicals between the two methods of animal sacrifice. Therefore, rapid inactivation of brain enzymes is not necessary prior to analysis for catecholamines and serotonin in mouse whole brain.

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Evaluation of the necessity for rapid inactivation of brain enzymes prior to analysis of norepinephrine dopamine and serotonin in the mouse

Abstract

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