Evaluation of the microsomal glutathione S-transferase 3 (MGST3) locus on 1q23 as a Type 2 diabetes susceptibility gene in Pima Indians

Farook Thameem, Xiaolin Yang, Paska A. Permana, Johanna K. Wolford, Clifton Bogardus, Michal Prochazka

Research output: Contribution to journalArticle

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Abstract

Elevation of plasma glucose concentration may induce generation of oxygen-free radicals, which can play an important role in the progression of diabetes and/or development of its complications. Various glutathione transferases utilize the availability of reduced glutathione for the cellular defense against oxygen-free radicals. One such enzyme is microsomal glutathione S-transferase 3 encoded by MGST3, which maps to chromosome 1q23, a region linked to Type 2 diabetes mellitus (T2DM) in Pima Indians, Caucasian, and Chinese populations. We investigated the MGST3 gene as a potential susceptibility gene for T2DM by screening this locus for single nucleotide polymorphisms (SNPs) in diabetic and non-diabetic Pima Indians. We also measured the skeletal muscle MGST3 mRNA level by Real-Time (RT) PCR and its relationship with insulin action in non-diabetic individuals. We identified 25 diallelic variants, most of which, based on their genotypic concordance, could be divided into three distinct linkage disequilibrium (LD) groups. We genotyped unique representative SNPs in selected diabetic and non-diabetic Pima Indians and found no evidence for association with T2DM. Furthermore, inter-individual variation of skeletal muscle MGST3 mRNA was not correlated with differences in insulin action in non-diabetic subjects. We conclude that alterations of MGST3 are unlikely to contribute to T2DM or differences in insulin sensitivity in the Pima Indians.

Original languageEnglish (US)
Pages (from-to)353-358
Number of pages6
JournalHuman Genetics
Volume113
Issue number4
DOIs
StatePublished - Sep 1 2003
Externally publishedYes

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Potassium Iodide
Glutathione Transferase
Type 2 Diabetes Mellitus
Genes
Free Radicals
Single Nucleotide Polymorphism
Reactive Oxygen Species
Skeletal Muscle
Insulin
Messenger RNA
Linkage Disequilibrium
Glutathione
Insulin Resistance
Real-Time Polymerase Chain Reaction
Chromosomes
Glucose
Enzymes

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Evaluation of the microsomal glutathione S-transferase 3 (MGST3) locus on 1q23 as a Type 2 diabetes susceptibility gene in Pima Indians. / Thameem, Farook; Yang, Xiaolin; Permana, Paska A.; Wolford, Johanna K.; Bogardus, Clifton; Prochazka, Michal.

In: Human Genetics, Vol. 113, No. 4, 01.09.2003, p. 353-358.

Research output: Contribution to journalArticle

Thameem, Farook ; Yang, Xiaolin ; Permana, Paska A. ; Wolford, Johanna K. ; Bogardus, Clifton ; Prochazka, Michal. / Evaluation of the microsomal glutathione S-transferase 3 (MGST3) locus on 1q23 as a Type 2 diabetes susceptibility gene in Pima Indians. In: Human Genetics. 2003 ; Vol. 113, No. 4. pp. 353-358.
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