TY - JOUR
T1 - Evaluation of the genetic response of U937 and Jurkat cells to 10-nanosecond electrical pulses (nsEP)
AU - Roth, Caleb C.
AU - Glickman, Randolph D.
AU - Tolstykh, Gleb P.
AU - Estlack, Larry E.
AU - Moen, Erick K.
AU - Echchgadda, Ibtissam
AU - Beier, Hope T.
AU - Barnes, Ronald A.
AU - Ibey, Bennett L.
N1 - Funding Information:
Mr. Roth is a SMART Scholar and is supported by the OSD-T&E (Office of Secretary Defense-Test and Evaluation), Defense–Wide / PE0601120D8Z National Defense Education Program (NDEP) / BA-1, Basic Research.
Publisher Copyright:
© 2016, Public Library of Science. All rights reserved. This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Nanosecond electrical pulse (nsEP) exposure activates signaling pathways, produces oxidative stress, stimulates hormone secretion, causes cell swelling and induces apoptotic and necrotic death. The underlying biophysical connection(s) between these diverse cellular reactions and nsEP has yet to be elucidated. Using global genetic analysis, we evaluated how two commonly studied cell types, U937 and Jurkat, respond to nsEP exposure. We hypothesized that by studying the genetic response of the cells following exposure, we would gain direct insight into the stresses experienced by the cell and in turn better understand the biophysical interaction taking place during the exposure. Using Ingenuity Systems software, we found genes associated with cell growth, movement and development to be significantly up-regulated in both cell types 4 h post exposure to nsEP. In agreement with our hypothesis, we also found that both cell lines exhibit significant biological changes consistent with mechanical stress induction. These results advance nsEP research by providing strong evidence that the interaction of nsEPs with cells involves mechanical stress.
AB - Nanosecond electrical pulse (nsEP) exposure activates signaling pathways, produces oxidative stress, stimulates hormone secretion, causes cell swelling and induces apoptotic and necrotic death. The underlying biophysical connection(s) between these diverse cellular reactions and nsEP has yet to be elucidated. Using global genetic analysis, we evaluated how two commonly studied cell types, U937 and Jurkat, respond to nsEP exposure. We hypothesized that by studying the genetic response of the cells following exposure, we would gain direct insight into the stresses experienced by the cell and in turn better understand the biophysical interaction taking place during the exposure. Using Ingenuity Systems software, we found genes associated with cell growth, movement and development to be significantly up-regulated in both cell types 4 h post exposure to nsEP. In agreement with our hypothesis, we also found that both cell lines exhibit significant biological changes consistent with mechanical stress induction. These results advance nsEP research by providing strong evidence that the interaction of nsEPs with cells involves mechanical stress.
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U2 - 10.1371/journal.pone.0154555
DO - 10.1371/journal.pone.0154555
M3 - Article
C2 - 27135944
AN - SCOPUS:84966708271
VL - 11
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 5
M1 - e0154555
ER -