Evaluation of the correlation of caspofungin MICs and treatment outcome in murine infections by wild type strains of Candida parapsilosis

Valentina Salas, F. Javier Pastor, Javier Capilla, Deanna A. Sutton, Emilio Mayayo, Annette W. Fothergill, Michael G. Rinaldi, Josep Guarro

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We have evaluated the in vitro activity of caspofungin against 36 wild-type strains of Candida parapsilosis sensu stricto using 3 techniques: broth microdilution, disk diffusion, and the determination of minimal fungicidal concentration (MFC). The first 2 methods showed a good in vitro activity of caspofungin, but the MFCs were ≥2 dilutions above their corresponding MICs. In a murine model of disseminated infection, we evaluated the efficacy of caspofungin at 5 mg/kg against 8 strains of C. parapsilosis representing different degrees of in vitro susceptibility (0.12-1 μg/mL). All the isolates responded to treatment and (1→3)-β-D-glucan levels were reduced in all the cases; however, the study revealed differences among isolates, since caspofungin reduced the tissue burden of mice infected with isolates with MICs ≤0.5 μg/mL but was less effective against those with MICs of 1 μg/mL.

Original languageEnglish (US)
Pages (from-to)41-45
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume77
Issue number1
DOIs
StatePublished - Sep 1 2013

Keywords

  • Animal models
  • C. parapsilosis
  • Caspofungin
  • Fungal infections

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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