Evaluation of RepliVAX WN, a single-cycle flavivirus vaccine, in a non-human primate model of west nile virus infection

Douglas G. Widman, Tomohiro Ishikawa, Luis D. Giavedoni, Vida L. Hodara, Melissa De La Garza, Jessica A. Montalbo, Amelia P. Travassos Da Rosa, Robert B. Tesh, Jean L. Patterson, Ricardo Carrion, Nigel Bourne, Peter W. Mason

    Research output: Contribution to journalArticle

    28 Scopus citations

    Abstract

    West Nile virus (WNV) causes serious neurologic disease, but no licensed vaccines are available to prevent this disease in humans. We have developed RepliVAX WN, a single-cycle flavivirus with an expected safety profile superior to other types of live-attenuated viral vaccines. In this report we describe studies examining RepliVAX WN safety, potency, and efficacy in a non-human primate model of WNV infection. A single immunization of four rhesus macaques with RepliVAX WN was safe and elicited detectable neutralizing antibody titers and IgM and IgG responses, and IgG titers were increased in two animals that received a second immunization. After challenge with WNV, three of four immunized animals were completely protected from viremia, and the remaining animal showed minimal viremia on one day. In contrast, the unvaccinated animal developed viremia that lasted six days. These results demonstrate the efficacy and safety of RepliVAX WN in this primate model of WNV infection.

    Original languageEnglish (US)
    Pages (from-to)1160-1167
    Number of pages8
    JournalAmerican Journal of Tropical Medicine and Hygiene
    Volume82
    Issue number6
    DOIs
    StatePublished - Jun 2010

    ASJC Scopus subject areas

    • Parasitology
    • Virology
    • Infectious Diseases

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    Widman, D. G., Ishikawa, T., Giavedoni, L. D., Hodara, V. L., De La Garza, M., Montalbo, J. A., Travassos Da Rosa, A. P., Tesh, R. B., Patterson, J. L., Carrion, R., Bourne, N., & Mason, P. W. (2010). Evaluation of RepliVAX WN, a single-cycle flavivirus vaccine, in a non-human primate model of west nile virus infection. American Journal of Tropical Medicine and Hygiene, 82(6), 1160-1167. https://doi.org/10.4269/ajtmh.2010.09-0310