Evaluation of humoral response to tumor antigens using recombinant expression-based serological mini-arrays (SMARTA)

Maria A. Lagarkova, Ekaterina P. Koroleva, Dmitry V. Kuprash, Veronica E. Boitchenko, Ulyana A. Kashkarova, Sergei A. Nedospasov, Yuriy V. Shebzukhov

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Screening of expression cDNA libraries derived from human neoplasms with autologous sera (SEREX) is an established method for defining antigens immunogenic in individual cancer patients. Although the majority of SEREX-derived cDNA clones encode autoantigens, some of them represent shared cancer antigens with cancer-related serological profiles. Routine evaluation of multiple SEREX-derived clones in serological assays using panels of allogeneic sera from cancer patients is an important step towards defining disease parameters of diagnostic and prognostic significance. Here we show how the seroreactivity of multiple SEREX-derived antigens can be simultaneously evaluated using a rapid semi-quantitative protocol of allogeneic screening, which we call SMARTA (serological mini-arrays of recombinant tumor antigens).

Original languageEnglish (US)
Pages (from-to)71-74
Number of pages4
JournalImmunology Letters
Volume85
Issue number1
DOIs
StatePublished - Jan 2 2003
Externally publishedYes

Keywords

  • Cancer antigens
  • Humoral response
  • Immunoscreening

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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