Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples

Humoral Response Potentially Implicated in Modulating Transmission Rates

Mari´a Julia Ruiz, Jimena Salido, Lorena Abusamra, Yanina Ghiglione, Cintia Cevallos, Gabriel Damilano, Ana Mari´a Rodriguez, Ce´sar Trifone, Natalia Laufer, Luis D. Giavedoni, Omar Sued, Horacio Salomo´n, Mari´a Magdalena Gherardi, Gabriela Turk

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

As the HIV/AIDS pandemic still progresses, understanding the mechanisms governing viral transmission as well as protection from HIV acquisition is fundamental. In this context, cohorts of HIV serodiscordant heterosexual couples (SDC) represent a unique tool. The present study was aimed to evaluate specific parameters of innate, cellular and humoral immune responses in SDC. Specifically, plasma levels of cytokines and chemokines, HIV-specific T-cell responses, gp120-specific IgG and IgA antibodies, and HIV-specific antibody-dependent cellular cytotoxicity (ADCC) activity were assessed in nine HIV-exposed seronegative individuals (ESN) and their corresponding HIV seropositive partners (HIV+-P), in eighteen chronically infected HIV subjects (C), nine chronically infected subjects known to be HIV transmitters (CT) and ten healthy HIV- donors (HD). Very low magnitude HIV-specific cellular responses were found in two out of six ESN. Interestingly, HIV+-P had the highest ADCC magnitude, the lowest IgA levels and the highest IgG/IgA ratio, all compared to CT. Positive correlations between CD4+ T-cell counts and both IgG/IgA ratios and %ADCC killing uniquely distinguished HIV+-P. Additionally, evidence of IgA interference with ADCC responses from HIV+-P and CT is provided. These data suggest for the first time a potential role of ADCC and/or gp120-specific IgG/IgA balance in modulating heterosexual transmission. In sum, this study provides key information to understand the host factors that influence viral transmission, which should be considered in both the development of prophylactic vaccines and novel immunotherapies for HIV-1 infection.

Original languageEnglish (US)
JournalEBioMedicine
DOIs
StateAccepted/In press - Jul 10 2017
Externally publishedYes

Fingerprint

Heterosexuality
Humoral Immunity
Cellular Immunity
Immunoglobulin A
Cytotoxicity
HIV
Immunoglobulin G
Antibodies
T-cells
HIV Antibodies
Chemokines
Transmitters
Vaccines
Cytokines
Plasmas
T-Lymphocytes
Pandemics
CD4 Lymphocyte Count
Innate Immunity
Immunotherapy

Keywords

  • ADCC
  • HIV-1
  • HIV-1 transmission
  • Serodiscordant couples

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples : Humoral Response Potentially Implicated in Modulating Transmission Rates. / Ruiz, Mari´a Julia; Salido, Jimena; Abusamra, Lorena; Ghiglione, Yanina; Cevallos, Cintia; Damilano, Gabriel; Rodriguez, Ana Mari´a; Trifone, Ce´sar; Laufer, Natalia; Giavedoni, Luis D.; Sued, Omar; Salomo´n, Horacio; Gherardi, Mari´a Magdalena; Turk, Gabriela.

In: EBioMedicine, 10.07.2017.

Research output: Contribution to journalArticle

Ruiz, MJ, Salido, J, Abusamra, L, Ghiglione, Y, Cevallos, C, Damilano, G, Rodriguez, AM, Trifone, C, Laufer, N, Giavedoni, LD, Sued, O, Salomo´n, H, Gherardi, MM & Turk, G 2017, 'Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transmission Rates', EBioMedicine. https://doi.org/10.1016/j.ebiom.2017.11.001
Ruiz, Mari´a Julia ; Salido, Jimena ; Abusamra, Lorena ; Ghiglione, Yanina ; Cevallos, Cintia ; Damilano, Gabriel ; Rodriguez, Ana Mari´a ; Trifone, Ce´sar ; Laufer, Natalia ; Giavedoni, Luis D. ; Sued, Omar ; Salomo´n, Horacio ; Gherardi, Mari´a Magdalena ; Turk, Gabriela. / Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples : Humoral Response Potentially Implicated in Modulating Transmission Rates. In: EBioMedicine. 2017.
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abstract = "As the HIV/AIDS pandemic still progresses, understanding the mechanisms governing viral transmission as well as protection from HIV acquisition is fundamental. In this context, cohorts of HIV serodiscordant heterosexual couples (SDC) represent a unique tool. The present study was aimed to evaluate specific parameters of innate, cellular and humoral immune responses in SDC. Specifically, plasma levels of cytokines and chemokines, HIV-specific T-cell responses, gp120-specific IgG and IgA antibodies, and HIV-specific antibody-dependent cellular cytotoxicity (ADCC) activity were assessed in nine HIV-exposed seronegative individuals (ESN) and their corresponding HIV seropositive partners (HIV+-P), in eighteen chronically infected HIV subjects (C), nine chronically infected subjects known to be HIV transmitters (CT) and ten healthy HIV- donors (HD). Very low magnitude HIV-specific cellular responses were found in two out of six ESN. Interestingly, HIV+-P had the highest ADCC magnitude, the lowest IgA levels and the highest IgG/IgA ratio, all compared to CT. Positive correlations between CD4+ T-cell counts and both IgG/IgA ratios and {\%}ADCC killing uniquely distinguished HIV+-P. Additionally, evidence of IgA interference with ADCC responses from HIV+-P and CT is provided. These data suggest for the first time a potential role of ADCC and/or gp120-specific IgG/IgA balance in modulating heterosexual transmission. In sum, this study provides key information to understand the host factors that influence viral transmission, which should be considered in both the development of prophylactic vaccines and novel immunotherapies for HIV-1 infection.",
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AU - Abusamra, Lorena

AU - Ghiglione, Yanina

AU - Cevallos, Cintia

AU - Damilano, Gabriel

AU - Rodriguez, Ana Mari´a

AU - Trifone, Ce´sar

AU - Laufer, Natalia

AU - Giavedoni, Luis D.

AU - Sued, Omar

AU - Salomo´n, Horacio

AU - Gherardi, Mari´a Magdalena

AU - Turk, Gabriela

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AB - As the HIV/AIDS pandemic still progresses, understanding the mechanisms governing viral transmission as well as protection from HIV acquisition is fundamental. In this context, cohorts of HIV serodiscordant heterosexual couples (SDC) represent a unique tool. The present study was aimed to evaluate specific parameters of innate, cellular and humoral immune responses in SDC. Specifically, plasma levels of cytokines and chemokines, HIV-specific T-cell responses, gp120-specific IgG and IgA antibodies, and HIV-specific antibody-dependent cellular cytotoxicity (ADCC) activity were assessed in nine HIV-exposed seronegative individuals (ESN) and their corresponding HIV seropositive partners (HIV+-P), in eighteen chronically infected HIV subjects (C), nine chronically infected subjects known to be HIV transmitters (CT) and ten healthy HIV- donors (HD). Very low magnitude HIV-specific cellular responses were found in two out of six ESN. Interestingly, HIV+-P had the highest ADCC magnitude, the lowest IgA levels and the highest IgG/IgA ratio, all compared to CT. Positive correlations between CD4+ T-cell counts and both IgG/IgA ratios and %ADCC killing uniquely distinguished HIV+-P. Additionally, evidence of IgA interference with ADCC responses from HIV+-P and CT is provided. These data suggest for the first time a potential role of ADCC and/or gp120-specific IgG/IgA balance in modulating heterosexual transmission. In sum, this study provides key information to understand the host factors that influence viral transmission, which should be considered in both the development of prophylactic vaccines and novel immunotherapies for HIV-1 infection.

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