Evaluation of cytotoxic agents in human colonic tumor xenografts and mouse gastrointestinal tissues using a 3H-thymidine fractional incorporation assay

J. A. Houghton, Peter J Houghton

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9 Citations (Scopus)

Abstract

Drug induced changes in 3H-thymidine fractional incorporation (TFI) in 4 human colonic xenograft lines and mouse gastrointestinal tissues have been related to the growth inhibition and host toxicity respectively. Using the TFI assay, the combinations of actinomycin D with cyclophosphamide and methyl CCNU with pentamethyl-melamine have been examined. Both combinations showed greater than additive activity against only 1 of the 3 xenograft lines in which they were tested. The TFI assay allows more rapid evaluation of drug activity and is more sensitive than growth delay studies. It also allows quantitative measurement of cytotoxic activity in gastrointestinal tissues after sublethal dose levels.

Original languageEnglish (US)
Pages (from-to)763-769
Number of pages7
JournalEuropean Journal of Cancer (1965)
Volume15
Issue number5
DOIs
StatePublished - Jan 1 1979
Externally publishedYes

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Cytotoxins
Heterografts
Thymidine
Semustine
Neoplasms
Drug Evaluation
Dactinomycin
Growth
Cyclophosphamide
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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