Evaluation of antioxidant activity and characterization of phenolic constituents of Phyllanthus amarus root

Soumya Maity, Suchandra Chatterjee, Prasad Shekhar Variyar, Arun Sharma, Soumyakanti Adhikari, Santasree Mazumder

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

The antioxidant property of the 70% aqueous ethanol extract of Phyllanthus amarus roots and its ether-soluble, ethyl acetate-soluble, and aqueous fractions were investigated by various in vitro assays. The root extracts showed higher DPPH, hydroxyl, superoxide, and nitric oxide radical scavenging and reducing power activity. Among all the samples, the ethyl acetate-soluble fraction demonstrated highest radical scavenging activity and total phenolics content. Twenty-eight different phenolic compounds were identified by LCMS/MS analysis of the ethyl acetate-soluble fraction. The majority of the compounds were found to exist as their glycosides, and many of these were gallic acid derivatives. Free epicatechin and gallic acid were also identified in the ethyl acetate-soluble fraction. The present investigation suggested that P. amarus root is a potent antioxidant and can be used for the prevention of diseases related to oxidative stress.

Original languageEnglish (US)
Pages (from-to)3443-3450
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number14
DOIs
StatePublished - Apr 10 2013

Keywords

  • LCMS/MS analysis
  • Phyllanthus amarus
  • antioxidant activity
  • gallic acid
  • phenolic constituents

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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