Evaluation of a real-time fluorescent PCR assay for rapid detection of Group B Streptococci in neonatal blood

Stephen M. Golden; David M. Stamilio; Brian M. Faux; Wilfred P. Dela Cruz; Craig T. Shoemaker; Camille L. Blackmon; Sarah D. Stassen; Velvet M. Clark; James W. Smith; Oswald L. Johnson

doi:10.1016/j.diagmicrobio.2004.04.021

Evaluation of a real-time fluorescent PCR assay for rapid detection of Group B Streptococci in neonatal blood

Stephen M. Golden, David M. Stamilio, Brian M. Faux, Wilfred P. Dela Cruz, Craig T. Shoemaker, Camille L. Blackmon, Sarah D. Stassen, Velvet M. Clark, James W. Smith, Oswald L. Johnson

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Streptococcus agalactiae (Group B Streptococcus: GBS) is the major causative agent of neonatal sepsis. Neonates at risk for GBS infections are empirically administered broad-spectrum antibiotics for at least 48 h pending blood culture results. A rapid assay to expedite detection of GBS would facilitate initiation of specific antibiotic therapy. Conversely, expeditious proof of absence of infection will avoid unnecessary antibiotic use. Using the LightCycler™, we evaluated a hybridization probe polymerase chain reaction (PCR) assay to detect GBS-specific cfb gene target DNA sequence in blood specimens. Both sensitivity and specificity of the real-time PCR assay was 100%. The assay demonstrated 100% specificity when tested against 26 non-GBS bacteria. This method is capable of detecting as few as ∼100 copies or 10 pg of GBS genomic DNA. This real-time PCR method is rapid, sensitive, and specific for the detection of GBS in neonatal blood samples and holds great promise in its utility in the diagnostic laboratory.

Original language	English (US)
Pages (from-to)	7-13
Number of pages	7
Journal	Diagnostic Microbiology and Infectious Disease
Volume	50
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2004.04.021
State	Published - Sep 2004
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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Golden, S. M., Stamilio, D. M., Faux, B. M., Dela Cruz, W. P., Shoemaker, C. T., Blackmon, C. L., Stassen, S. D., Clark, V. M., Smith, J. W., & Johnson, O. L. (2004). Evaluation of a real-time fluorescent PCR assay for rapid detection of Group B Streptococci in neonatal blood. Diagnostic Microbiology and Infectious Disease, 50(1), 7-13. https://doi.org/10.1016/j.diagmicrobio.2004.04.021

Golden, SM, Stamilio, DM, Faux, BM, Dela Cruz, WP, Shoemaker, CT, Blackmon, CL, Stassen, SD, Clark, VM, Smith, JW & Johnson, OL 2004, 'Evaluation of a real-time fluorescent PCR assay for rapid detection of Group B Streptococci in neonatal blood', Diagnostic Microbiology and Infectious Disease, vol. 50, no. 1, pp. 7-13. https://doi.org/10.1016/j.diagmicrobio.2004.04.021

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abstract = "Streptococcus agalactiae (Group B Streptococcus: GBS) is the major causative agent of neonatal sepsis. Neonates at risk for GBS infections are empirically administered broad-spectrum antibiotics for at least 48 h pending blood culture results. A rapid assay to expedite detection of GBS would facilitate initiation of specific antibiotic therapy. Conversely, expeditious proof of absence of infection will avoid unnecessary antibiotic use. Using the LightCycler{\texttrademark}, we evaluated a hybridization probe polymerase chain reaction (PCR) assay to detect GBS-specific cfb gene target DNA sequence in blood specimens. Both sensitivity and specificity of the real-time PCR assay was 100%. The assay demonstrated 100% specificity when tested against 26 non-GBS bacteria. This method is capable of detecting as few as ∼100 copies or 10 pg of GBS genomic DNA. This real-time PCR method is rapid, sensitive, and specific for the detection of GBS in neonatal blood samples and holds great promise in its utility in the diagnostic laboratory.",

author = "Golden, {Stephen M.} and Stamilio, {David M.} and Faux, {Brian M.} and {Dela Cruz}, {Wilfred P.} and Shoemaker, {Craig T.} and Blackmon, {Camille L.} and Stassen, {Sarah D.} and Clark, {Velvet M.} and Smith, {James W.} and Johnson, {Oswald L.}",

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AU - Faux, Brian M.

AU - Dela Cruz, Wilfred P.

AU - Shoemaker, Craig T.

AU - Blackmon, Camille L.

AU - Stassen, Sarah D.

AU - Clark, Velvet M.

AU - Smith, James W.

AU - Johnson, Oswald L.

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AB - Streptococcus agalactiae (Group B Streptococcus: GBS) is the major causative agent of neonatal sepsis. Neonates at risk for GBS infections are empirically administered broad-spectrum antibiotics for at least 48 h pending blood culture results. A rapid assay to expedite detection of GBS would facilitate initiation of specific antibiotic therapy. Conversely, expeditious proof of absence of infection will avoid unnecessary antibiotic use. Using the LightCycler™, we evaluated a hybridization probe polymerase chain reaction (PCR) assay to detect GBS-specific cfb gene target DNA sequence in blood specimens. Both sensitivity and specificity of the real-time PCR assay was 100%. The assay demonstrated 100% specificity when tested against 26 non-GBS bacteria. This method is capable of detecting as few as ∼100 copies or 10 pg of GBS genomic DNA. This real-time PCR method is rapid, sensitive, and specific for the detection of GBS in neonatal blood samples and holds great promise in its utility in the diagnostic laboratory.

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Evaluation of a real-time fluorescent PCR assay for rapid detection of Group B Streptococci in neonatal blood

Abstract

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