@inproceedings{6e0a0d2f193f4638894b30f38f88c40e,
title = "Evaluation of a novel label-free photonic-crystal biosensor imaging system for the detection of prostate cancer cells",
abstract = "Biomarker screening for prostate-specific antigen (PSA) is the current clinical standard for detection of prostate cancer. However this method has shown many limitations, mainly in its specificity, which can lead to a high false positive rate. Thus, there is a growing need in developing a more specific detection system for prostate cancer. Using a Photonic- Crystal-based biosensor in a Total-Internal-Reflection (PC-TIR) configuration, we demonstrate the use of refractive index (RI) to accomplish label-free detection of prostate cancer cells against non-cancerous prostate epithelial cells. The PC-TIR biosensor possesses an open microcavity, which in contrast to traditional closed microcavities, allows for easier access of analyte molecules or cells to interact with its sensing surface. In this study, an imaging system was designed using the PC-TIR biosensor to quantify cell RI as the contrast parameter for prostate cancer detection. Non-cancerous BPH-1 prostate epithelial cells and prostate cancer PC-3 cells were placed on a single biosensor and measured concurrently. Recorded image data was then analyzed through a home-built MatLab program. Results demonstrate that RI is a suitable variable for differentiation between prostate cancer cells and non-cancerous prostate epithelial cells. Our study shows clinical potential in utilizing RI test for the detection of prostate cancer.",
keywords = "Cellular refractive index (RI), Label-free imaging system, Optical biosensors, PC-TIR, Photonic crystal, Prostate cancer",
author = "Frank DeLuna and Ding, {Xiao Fei} and Luzhe Sun and Ye, {Jing Yong}",
note = "Publisher Copyright: {\textcopyright} 2017 SPIE.; Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV ; Conference date: 30-01-2017 Through 01-02-2017",
year = "2017",
doi = "10.1117/12.2249630",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Farkas, {Daniel L.} and Nicolau, {Dan V.} and Leif, {Robert C.}",
booktitle = "Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV",
}