Evaluation of a novel label-free photonic-crystal biosensor imaging system for the detection of prostate cancer cells

Frank DeLuna, Xiao Fei Ding, Luzhe Sun, Jing Yong Ye

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Scopus citations

Abstract

Biomarker screening for prostate-specific antigen (PSA) is the current clinical standard for detection of prostate cancer. However this method has shown many limitations, mainly in its specificity, which can lead to a high false positive rate. Thus, there is a growing need in developing a more specific detection system for prostate cancer. Using a Photonic- Crystal-based biosensor in a Total-Internal-Reflection (PC-TIR) configuration, we demonstrate the use of refractive index (RI) to accomplish label-free detection of prostate cancer cells against non-cancerous prostate epithelial cells. The PC-TIR biosensor possesses an open microcavity, which in contrast to traditional closed microcavities, allows for easier access of analyte molecules or cells to interact with its sensing surface. In this study, an imaging system was designed using the PC-TIR biosensor to quantify cell RI as the contrast parameter for prostate cancer detection. Non-cancerous BPH-1 prostate epithelial cells and prostate cancer PC-3 cells were placed on a single biosensor and measured concurrently. Recorded image data was then analyzed through a home-built MatLab program. Results demonstrate that RI is a suitable variable for differentiation between prostate cancer cells and non-cancerous prostate epithelial cells. Our study shows clinical potential in utilizing RI test for the detection of prostate cancer.

Original languageEnglish (US)
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV
PublisherSPIE
Volume10068
ISBN (Electronic)9781510605770
DOIs
StatePublished - Jan 1 2017
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV - San Francisco, United States
Duration: Jan 30 2017Feb 1 2017

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV
CountryUnited States
CitySan Francisco
Period1/30/172/1/17

Keywords

  • Cellular refractive index (RI)
  • Label-free imaging system
  • Optical biosensors
  • PC-TIR
  • Photonic crystal
  • Prostate cancer

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    DeLuna, F., Ding, X. F., Sun, L., & Ye, J. Y. (2017). Evaluation of a novel label-free photonic-crystal biosensor imaging system for the detection of prostate cancer cells. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV (Vol. 10068). [100681H] SPIE. https://doi.org/10.1117/12.2249630