Ethyl acetate fraction of Eclipta alba: a potential phytopharmaceutical targeting adipocyte differentiation

Abhishek Gupta, Ashok Kumar, Durgesh Kumar, Shiv Nandan, Kripa Shankar, Salil Varshney, Sujith Rajan, Ankita Srivastava, Sanchita Gupta, Sanjeev Kanojiya, T. Narender, Anil Nilkanth Gaikwad

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Natural products have always fascinated mankind for their miraculous properties. Eclipta alba (E. alba), a medicinal herb has long been used in traditional medicine for curing several pathologies. It has been shown to have anti-diabetic effect as well as hepato-protective activity. Here, in order to address metabolic derangements, the study was designed to evaluate the efficacy of E. alba and its fractions in adipogenesis inhibition and dyslipidemia. Of the crude extract and fractions screened, ethyl acetate fraction of E. alba inhibited adipocyte differentiation in 3T3-L1 pre-adipocytes and hMSC derived adipocytes. It inhibited mitotic clonal expansion and caused cell cycle arrest in G1 and S phase as suggested by western blot analysis and flow cytometry. It was also shown to have lipolytic effects. Oral administration of ethyl acetate fraction of E. alba to hamsters unveiled its anti-adipogenic as well as anti-dyslipidemic activity in-vivo. Mass spectrometry analysis of ethyl acetate fraction confirmed the presence of several bioactive components, projecting it as an effective phytopharmaceutical agent. In conclusion, ethyl acetate fraction of E. alba possesses potent anti-adipogenic as well as anti-dyslipidemic activity and could be projected as an herbal formulation towards obesity.

Original languageEnglish (US)
Pages (from-to)572-583
Number of pages12
JournalBiomedicine and Pharmacotherapy
Volume96
DOIs
StatePublished - Dec 2017
Externally publishedYes

Keywords

  • 3T3-L1 adipocyte
  • Adipogenesis
  • Dyslipidemia

ASJC Scopus subject areas

  • Pharmacology

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