Ethanol-mediated inhibition of mitogen-activated protein kinase phosphorylation in mouse brain

Haviryaji S.G. Kalluri, Maharaj K. Ticku

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Ethanol (1.5-3.5 g/kg body weight) was administered intraperitoneally to mice and the phosphorylation of MAP (mitogen-activated protein) kinase in the cerebral cortex was determined using phospho-specific MAP kinase antibodies. Ethanol inhibited the phosphorylation of MAP kinase in a dose- and time-dependent manner. Developmental studies demonstrated that the levels of phospho-MAP kinase increased from fetal cortex (prenatal) to 16-day-old mice (postnatal) and remained constant up to 4 months of age. However, ethanol (3.5 g/kg) decreased the phospho-MAP kinase staining in all of the age groups studied. Subcellular fractionation studies demonstrated that ethanol inhibited the phosphorylation of MAP kinase in both the cytosol as well as nucleus, but did not alter the levels of MAP kinase. Likewise, MK-801 (0.4 mg/kg) or flurazepam (75 mg/kg) also decreased the phospho-MAP kinase content. These data indicate that ethanol may inhibit the phosphorylation of MAP kinase in vivo by either inhibiting NMDA receptors or activating GABA receptors.

Original languageEnglish (US)
Pages (from-to)53-58
Number of pages6
JournalEuropean Journal of Pharmacology
Volume439
Issue number1-3
DOIs
StatePublished - Mar 29 2002

Keywords

  • Cerebral cortex
  • Ethanol
  • MAP (mitogen-activated protein) kinase

ASJC Scopus subject areas

  • Pharmacology

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