Estrogen pretreatment protects males against hypoxia-induced immune depression

Markus W. Knöferl, Martin G Schwacha, Doraid Jarrar, Martin K. Angele, Keith Fragoza, Kirby I. Bland, Irshad H. Chaudry

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Hypoxemia depresses cell-mediated immune functions in males, whereas proestrous females do not show such a depression. We hypothesized that elevated systemic estradiol levels in proestrous females prevent hypoxemia-induced immune depression. To study this hypothesis, male C3H/HeN mice were pretreated with 17β-estradiol (E2, 40 μg/kg body wt sc) or vehicle for 3 days before induction of hypoxemia and again immediately before induction of hypoxia. The mice were subjected to hypoxemia (95% N2-5% 02) or sham hypoxemia (room air) for 60 min, and plasma and spleen cells were collected 2 h later. In vehicle-treated mice, splenocyte proliferation and interleukin-2 and interleukin-3 production were depressed after hypoxemia. E2-pretreated animals, however, displayed no such depression in splenic T cell parameters after hypoxemia. Splenic macrophage cytokine production was also depressed in vehicle-treated mice subjected to hypoxia, whereas it was normal in E2-pretreated mice. In summary, these findings indicate that administration of E2 before hypoxemia prevented the depression of cell-mediated immune functions. Thus administration of 17β-estradiol in high-risk patients before major surgery might decrease hypoxemia-induced immune depression under those conditions.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume282
Issue number5 51-5
StatePublished - 2002
Externally publishedYes

Fingerprint

Estradiol
Estrogens
T-cells
Macrophages
Interleukin-3
Surgery
Interleukin-2
Animals
Cytokines
Plasmas
Air
Hypoxia
Inbred C3H Mouse
Plasma Cells
Spleen
T-Lymphocytes

Keywords

  • Cytokines
  • Estradiol
  • Gender
  • Inflammation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Knöferl, M. W., Schwacha, M. G., Jarrar, D., Angele, M. K., Fragoza, K., Bland, K. I., & Chaudry, I. H. (2002). Estrogen pretreatment protects males against hypoxia-induced immune depression. American Journal of Physiology - Cell Physiology, 282(5 51-5).

Estrogen pretreatment protects males against hypoxia-induced immune depression. / Knöferl, Markus W.; Schwacha, Martin G; Jarrar, Doraid; Angele, Martin K.; Fragoza, Keith; Bland, Kirby I.; Chaudry, Irshad H.

In: American Journal of Physiology - Cell Physiology, Vol. 282, No. 5 51-5, 2002.

Research output: Contribution to journalArticle

Knöferl, MW, Schwacha, MG, Jarrar, D, Angele, MK, Fragoza, K, Bland, KI & Chaudry, IH 2002, 'Estrogen pretreatment protects males against hypoxia-induced immune depression', American Journal of Physiology - Cell Physiology, vol. 282, no. 5 51-5.
Knöferl, Markus W. ; Schwacha, Martin G ; Jarrar, Doraid ; Angele, Martin K. ; Fragoza, Keith ; Bland, Kirby I. ; Chaudry, Irshad H. / Estrogen pretreatment protects males against hypoxia-induced immune depression. In: American Journal of Physiology - Cell Physiology. 2002 ; Vol. 282, No. 5 51-5.
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