Abstract
Hypoxemia depresses cell-mediated immune functions in males, whereas proestrous females do not show such a depression. We hypothesized that elevated systemic estradiol levels in proestrous females prevent hypoxemia-induced immune depression. To study this hypothesis, male C3H/HeN mice were pretreated with 17β-estradiol (E2, 40 μg/kg body wt sc) or vehicle for 3 days before induction of hypoxemia and again immediately before induction of hypoxia. The mice were subjected to hypoxemia (95% N2-5% 02) or sham hypoxemia (room air) for 60 min, and plasma and spleen cells were collected 2 h later. In vehicle-treated mice, splenocyte proliferation and interleukin-2 and interleukin-3 production were depressed after hypoxemia. E2-pretreated animals, however, displayed no such depression in splenic T cell parameters after hypoxemia. Splenic macrophage cytokine production was also depressed in vehicle-treated mice subjected to hypoxia, whereas it was normal in E2-pretreated mice. In summary, these findings indicate that administration of E2 before hypoxemia prevented the depression of cell-mediated immune functions. Thus administration of 17β-estradiol in high-risk patients before major surgery might decrease hypoxemia-induced immune depression under those conditions.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Cell Physiology |
| Volume | 282 |
| Issue number | 5 51-5 |
| State | Published - 2002 |
| Externally published | Yes |
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Keywords
- Cytokines
- Estradiol
- Gender
- Inflammation
ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology
- Physiology
Cite this
Estrogen pretreatment protects males against hypoxia-induced immune depression. / Knöferl, Markus W.; Schwacha, Martin G; Jarrar, Doraid; Angele, Martin K.; Fragoza, Keith; Bland, Kirby I.; Chaudry, Irshad H.
In: American Journal of Physiology - Cell Physiology, Vol. 282, No. 5 51-5, 2002.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Estrogen pretreatment protects males against hypoxia-induced immune depression
AU - Knöferl, Markus W.
AU - Schwacha, Martin G
AU - Jarrar, Doraid
AU - Angele, Martin K.
AU - Fragoza, Keith
AU - Bland, Kirby I.
AU - Chaudry, Irshad H.
PY - 2002
Y1 - 2002
N2 - Hypoxemia depresses cell-mediated immune functions in males, whereas proestrous females do not show such a depression. We hypothesized that elevated systemic estradiol levels in proestrous females prevent hypoxemia-induced immune depression. To study this hypothesis, male C3H/HeN mice were pretreated with 17β-estradiol (E2, 40 μg/kg body wt sc) or vehicle for 3 days before induction of hypoxemia and again immediately before induction of hypoxia. The mice were subjected to hypoxemia (95% N2-5% 02) or sham hypoxemia (room air) for 60 min, and plasma and spleen cells were collected 2 h later. In vehicle-treated mice, splenocyte proliferation and interleukin-2 and interleukin-3 production were depressed after hypoxemia. E2-pretreated animals, however, displayed no such depression in splenic T cell parameters after hypoxemia. Splenic macrophage cytokine production was also depressed in vehicle-treated mice subjected to hypoxia, whereas it was normal in E2-pretreated mice. In summary, these findings indicate that administration of E2 before hypoxemia prevented the depression of cell-mediated immune functions. Thus administration of 17β-estradiol in high-risk patients before major surgery might decrease hypoxemia-induced immune depression under those conditions.
AB - Hypoxemia depresses cell-mediated immune functions in males, whereas proestrous females do not show such a depression. We hypothesized that elevated systemic estradiol levels in proestrous females prevent hypoxemia-induced immune depression. To study this hypothesis, male C3H/HeN mice were pretreated with 17β-estradiol (E2, 40 μg/kg body wt sc) or vehicle for 3 days before induction of hypoxemia and again immediately before induction of hypoxia. The mice were subjected to hypoxemia (95% N2-5% 02) or sham hypoxemia (room air) for 60 min, and plasma and spleen cells were collected 2 h later. In vehicle-treated mice, splenocyte proliferation and interleukin-2 and interleukin-3 production were depressed after hypoxemia. E2-pretreated animals, however, displayed no such depression in splenic T cell parameters after hypoxemia. Splenic macrophage cytokine production was also depressed in vehicle-treated mice subjected to hypoxia, whereas it was normal in E2-pretreated mice. In summary, these findings indicate that administration of E2 before hypoxemia prevented the depression of cell-mediated immune functions. Thus administration of 17β-estradiol in high-risk patients before major surgery might decrease hypoxemia-induced immune depression under those conditions.
KW - Cytokines
KW - Estradiol
KW - Gender
KW - Inflammation
UR - http://www.scopus.com/inward/record.url?scp=0036086897&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036086897&partnerID=8YFLogxK
M3 - Article
C2 - 11940524
AN - SCOPUS:0036086897
VL - 282
JO - American Journal of Physiology - Renal Physiology
JF - American Journal of Physiology - Renal Physiology
SN - 0363-6127
IS - 5 51-5
ER -