Estrogen modulates neural-immune interactions through intracellular signaling pathways and antioxidant enzyme activity in the spleen of middle-aged ovariectomized female rats

Prathamesh Kale; Aparna Mohanty; Anushree Patil; Miti Mishra; Uday P. Pratap; Hannah P. Priyanka; Srinivasan ThyagaRajan

doi:10.1016/j.jneuroim.2013.11.003

Estrogen modulates neural-immune interactions through intracellular signaling pathways and antioxidant enzyme activity in the spleen of middle-aged ovariectomized female rats

Prathamesh Kale
, Aparna Mohanty
, Anushree Patil
, Miti Mishra
, Uday P. Pratap
, Hannah P. Priyanka
, Srinivasan ThyagaRajan

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Modulation of neural-immune interactions by estrogen in the spleens of ovariectomized (OVX) middle-aged female rats was examined. Con A-induced lymphoproliferation, splenic tyrosine hydroxylase (TH) and nerve growth factor (NGF) expression, levels of p-ERK 1/2, p-CREB, and p-Akt, and activity of superoxide dismutase decreased in OVX rats while estrogen treatment enhanced their expression, levels, and activity. Also, estrogen treatment enhanced Con A-induced IFN-γ production and decreased Con A-induced IL-2 production compared to OVX animals. In contrast, estrogen increased the extent of lipid peroxidation and protein carbonyl formation while OVX induced a decline in protein carbonyl formation. These results suggest that estrogen enhances neural-immune interactions while simultaneously affecting it through generation of free radicals as reflected by increased lipid peroxidation and protein carbonyl formation.

Original language	English (US)
Pages (from-to)	7-15
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	267
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2013.11.003
State	Published - 2014
Externally published	Yes

Keywords

Akt
CREB
Cytokine
ERK
Nerve growth factor
Tyrosine hydroxylase

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/j.jneuroim.2013.11.003

Cite this

Kale, P., Mohanty, A., Patil, A., Mishra, M., Pratap, U. P., Priyanka, H. P., & ThyagaRajan, S. (2014). Estrogen modulates neural-immune interactions through intracellular signaling pathways and antioxidant enzyme activity in the spleen of middle-aged ovariectomized female rats. Journal of Neuroimmunology, 267(1-2), 7-15. https://doi.org/10.1016/j.jneuroim.2013.11.003

Kale, P, Mohanty, A, Patil, A, Mishra, M, Pratap, UP, Priyanka, HP & ThyagaRajan, S 2014, 'Estrogen modulates neural-immune interactions through intracellular signaling pathways and antioxidant enzyme activity in the spleen of middle-aged ovariectomized female rats', Journal of Neuroimmunology, vol. 267, no. 1-2, pp. 7-15. https://doi.org/10.1016/j.jneuroim.2013.11.003

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title = "Estrogen modulates neural-immune interactions through intracellular signaling pathways and antioxidant enzyme activity in the spleen of middle-aged ovariectomized female rats",

abstract = "Modulation of neural-immune interactions by estrogen in the spleens of ovariectomized (OVX) middle-aged female rats was examined. Con A-induced lymphoproliferation, splenic tyrosine hydroxylase (TH) and nerve growth factor (NGF) expression, levels of p-ERK 1/2, p-CREB, and p-Akt, and activity of superoxide dismutase decreased in OVX rats while estrogen treatment enhanced their expression, levels, and activity. Also, estrogen treatment enhanced Con A-induced IFN-γ production and decreased Con A-induced IL-2 production compared to OVX animals. In contrast, estrogen increased the extent of lipid peroxidation and protein carbonyl formation while OVX induced a decline in protein carbonyl formation. These results suggest that estrogen enhances neural-immune interactions while simultaneously affecting it through generation of free radicals as reflected by increased lipid peroxidation and protein carbonyl formation.",

keywords = "Akt, CREB, Cytokine, ERK, Nerve growth factor, Tyrosine hydroxylase",

author = "Prathamesh Kale and Aparna Mohanty and Anushree Patil and Miti Mishra and Pratap, \{Uday P.\} and Priyanka, \{Hannah P.\} and Srinivasan ThyagaRajan",

note = "Funding Information: We thank Himanshu Sharma, Ran Vijay Singh, S. Gopinath, Vishak Raman, and Karthik Ramakrishnan for technical assistance. Supported by the Department of Science and Technology, Government of India, New Delhi (F. NO. SR/SO/HS-46/2007 ).",

year = "2014",

doi = "10.1016/j.jneuroim.2013.11.003",

language = "English (US)",

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AU - Kale, Prathamesh

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AU - Mishra, Miti

AU - Pratap, Uday P.

AU - Priyanka, Hannah P.

AU - ThyagaRajan, Srinivasan

N1 - Funding Information: We thank Himanshu Sharma, Ran Vijay Singh, S. Gopinath, Vishak Raman, and Karthik Ramakrishnan for technical assistance. Supported by the Department of Science and Technology, Government of India, New Delhi (F. NO. SR/SO/HS-46/2007 ).

PY - 2014

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N2 - Modulation of neural-immune interactions by estrogen in the spleens of ovariectomized (OVX) middle-aged female rats was examined. Con A-induced lymphoproliferation, splenic tyrosine hydroxylase (TH) and nerve growth factor (NGF) expression, levels of p-ERK 1/2, p-CREB, and p-Akt, and activity of superoxide dismutase decreased in OVX rats while estrogen treatment enhanced their expression, levels, and activity. Also, estrogen treatment enhanced Con A-induced IFN-γ production and decreased Con A-induced IL-2 production compared to OVX animals. In contrast, estrogen increased the extent of lipid peroxidation and protein carbonyl formation while OVX induced a decline in protein carbonyl formation. These results suggest that estrogen enhances neural-immune interactions while simultaneously affecting it through generation of free radicals as reflected by increased lipid peroxidation and protein carbonyl formation.

AB - Modulation of neural-immune interactions by estrogen in the spleens of ovariectomized (OVX) middle-aged female rats was examined. Con A-induced lymphoproliferation, splenic tyrosine hydroxylase (TH) and nerve growth factor (NGF) expression, levels of p-ERK 1/2, p-CREB, and p-Akt, and activity of superoxide dismutase decreased in OVX rats while estrogen treatment enhanced their expression, levels, and activity. Also, estrogen treatment enhanced Con A-induced IFN-γ production and decreased Con A-induced IL-2 production compared to OVX animals. In contrast, estrogen increased the extent of lipid peroxidation and protein carbonyl formation while OVX induced a decline in protein carbonyl formation. These results suggest that estrogen enhances neural-immune interactions while simultaneously affecting it through generation of free radicals as reflected by increased lipid peroxidation and protein carbonyl formation.

KW - Akt

KW - CREB

KW - Cytokine

KW - ERK

KW - Nerve growth factor

KW - Tyrosine hydroxylase

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Estrogen modulates neural-immune interactions through intracellular signaling pathways and antioxidant enzyme activity in the spleen of middle-aged ovariectomized female rats

Abstract

Keywords

ASJC Scopus subject areas

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