Estrogen effects on 5-HT1A receptors in hippocampal membranes from ovariectomized rats: functional and binding studies

William P. Clarke; Saul Maayani

doi:10.1016/0006-8993(90)90983-I

Estrogen effects on 5-HT_1A receptors in hippocampal membranes from ovariectomized rats: functional and binding studies

William P. Clarke, Saul Maayani

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Chronic treatment (4 days) of ovariectomized rats with estrogen produced a two-fold shift to left (with no change in the maximal percent inhibition) in the concentration response curve for the inhibition of adenylyl cyclase activity by 5-HT, but did not alter curves for R-PIA (adenosine A₁ agonist) or Gpp(NH)p (to activate G_i). Furthermore, estrogen treatment had no effect on the number or affinity of 5-HT_1A binding sites labeled with [³H]8-OH-DPAT. These data, when considered with the results of previous studies, suggest that estrogen treatment may selectively enhance 5-HT_1A-mediated responses in rat hippocampus.

Original language	English (US)
Pages (from-to)	287-291
Number of pages	5
Journal	Brain Research
Volume	518
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(90)90983-I
State	Published - Jun 4 1990
Externally published	Yes

Keywords

8-Hydroxy-2-(di-n-propylamine)tetralin
Adenosine A
Adenylyl cyclase
Female
Guanosine 5′-(β,γ-imino)triphosphate
Guanosine 5′-triphosphate-binding protein
Serotonin

ASJC Scopus subject areas

Clinical Neurology
Molecular Biology
General Neuroscience
Developmental Biology

Access to Document

10.1016/0006-8993(90)90983-I

Cite this

@article{49661aa247c54c0e87fd0f9a3786648c,

title = "Estrogen effects on 5-HT1A receptors in hippocampal membranes from ovariectomized rats: functional and binding studies",

abstract = "Chronic treatment (4 days) of ovariectomized rats with estrogen produced a two-fold shift to left (with no change in the maximal percent inhibition) in the concentration response curve for the inhibition of adenylyl cyclase activity by 5-HT, but did not alter curves for R-PIA (adenosine A1 agonist) or Gpp(NH)p (to activate Gi). Furthermore, estrogen treatment had no effect on the number or affinity of 5-HT1A binding sites labeled with [3H]8-OH-DPAT. These data, when considered with the results of previous studies, suggest that estrogen treatment may selectively enhance 5-HT1A-mediated responses in rat hippocampus.",

keywords = "8-Hydroxy-2-(di-n-propylamine)tetralin, Adenosine A, Adenylyl cyclase, Female, Guanosine 5′-(β,γ-imino)triphosphate, Guanosine 5′-triphosphate-binding protein, Serotonin",

author = "Clarke, {William P.} and Saul Maayani",

note = "Funding Information: This work was supported by United States Public Health Service Grants GM-34852 and DA-01875. W.P.C. is a Revson Fellow supported by an award from the Charles H. Revson Foundation. The authors would like to thank Kelly Ann Berg and Barbara Craddock-Royal for help with the adenylyl cyclase and binding assays.",

year = "1990",

month = jun,

day = "4",

doi = "10.1016/0006-8993(90)90983-I",

language = "English (US)",

volume = "518",

pages = "287--291",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier B.V.",

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T1 - Estrogen effects on 5-HT1A receptors in hippocampal membranes from ovariectomized rats

T2 - functional and binding studies

AU - Clarke, William P.

AU - Maayani, Saul

N1 - Funding Information: This work was supported by United States Public Health Service Grants GM-34852 and DA-01875. W.P.C. is a Revson Fellow supported by an award from the Charles H. Revson Foundation. The authors would like to thank Kelly Ann Berg and Barbara Craddock-Royal for help with the adenylyl cyclase and binding assays.

PY - 1990/6/4

Y1 - 1990/6/4

N2 - Chronic treatment (4 days) of ovariectomized rats with estrogen produced a two-fold shift to left (with no change in the maximal percent inhibition) in the concentration response curve for the inhibition of adenylyl cyclase activity by 5-HT, but did not alter curves for R-PIA (adenosine A1 agonist) or Gpp(NH)p (to activate Gi). Furthermore, estrogen treatment had no effect on the number or affinity of 5-HT1A binding sites labeled with [3H]8-OH-DPAT. These data, when considered with the results of previous studies, suggest that estrogen treatment may selectively enhance 5-HT1A-mediated responses in rat hippocampus.

AB - Chronic treatment (4 days) of ovariectomized rats with estrogen produced a two-fold shift to left (with no change in the maximal percent inhibition) in the concentration response curve for the inhibition of adenylyl cyclase activity by 5-HT, but did not alter curves for R-PIA (adenosine A1 agonist) or Gpp(NH)p (to activate Gi). Furthermore, estrogen treatment had no effect on the number or affinity of 5-HT1A binding sites labeled with [3H]8-OH-DPAT. These data, when considered with the results of previous studies, suggest that estrogen treatment may selectively enhance 5-HT1A-mediated responses in rat hippocampus.

KW - 8-Hydroxy-2-(di-n-propylamine)tetralin

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KW - Adenylyl cyclase

KW - Female

KW - Guanosine 5′-(β,γ-imino)triphosphate

KW - Guanosine 5′-triphosphate-binding protein

KW - Serotonin

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