Establishment of synchronization in carrot cell suspension culture and studies on stage specific activation of glyoxalase I

Sagar Ghosh, Jayanti Sen, Sanjeev Kalia, Sipra Guha-Mukherjee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The present report summarizes and compares the effects of three cell cycle inhibitors, viz. aphidicolin, hydroxyurea and mimosine, in inducing synchronization of a rapidly proliferating suspension culture of carrot. These treatments efficiently synchronized the cell cycle as the doubling time of the cell population was roughly equal to the total length of one cell cycle. Protoplasts derived from mimosine treated cell suspension culture were resolved via flow cytometry to get an idea of the temporal organization of the cell cycle events. The biochemical analysis showed a rise in stage specific activity of glyoxalase I, an auxin inducible marker enzyme activated at G2-M. This activity peak could be shifted to an early phase of interphase in response to auxin treatment.

Original languageEnglish (US)
Pages (from-to)141-148
Number of pages8
JournalMethods in Cell Science
Volume21
Issue number2-3
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Lactoylglutathione Lyase
Daucus carota
Suspensions
Cell Cycle
Synchronization
Mimosine
Cell Culture Techniques
Chemical activation
Cells
Indoleacetic Acids
Aphidicolin
Cell culture
Hydroxyurea
Protoplasts
Interphase
Flow Cytometry
Flow cytometry
Enzymes
Population

Keywords

  • Carrot cell suspension
  • Cell cycle study
  • Glyoxalase I activity
  • Inhibitors
  • Synchronization

ASJC Scopus subject areas

  • Cell Biology

Cite this

Establishment of synchronization in carrot cell suspension culture and studies on stage specific activation of glyoxalase I. / Ghosh, Sagar; Sen, Jayanti; Kalia, Sanjeev; Guha-Mukherjee, Sipra.

In: Methods in Cell Science, Vol. 21, No. 2-3, 1999, p. 141-148.

Research output: Contribution to journalArticle

Ghosh, Sagar ; Sen, Jayanti ; Kalia, Sanjeev ; Guha-Mukherjee, Sipra. / Establishment of synchronization in carrot cell suspension culture and studies on stage specific activation of glyoxalase I. In: Methods in Cell Science. 1999 ; Vol. 21, No. 2-3. pp. 141-148.
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