Establishment of synchronization in carrot cell suspension culture and studies on stage specific activation of glyoxalase I

Sagar Ghosh, Jayanti Sen, Sanjeev Kalia, Sipra Guha-Mukherjee

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The present report summarizes and compares the effects of three cell cycle inhibitors, viz. aphidicolin, hydroxyurea and mimosine, in inducing synchronization of a rapidly proliferating suspension culture of carrot. These treatments efficiently synchronized the cell cycle as the doubling time of the cell population was roughly equal to the total length of one cell cycle. Protoplasts derived from mimosine treated cell suspension culture were resolved via flow cytometry to get an idea of the temporal organization of the cell cycle events. The biochemical analysis showed a rise in stage specific activity of glyoxalase I, an auxin inducible marker enzyme activated at G2-M. This activity peak could be shifted to an early phase of interphase in response to auxin treatment.

Original languageEnglish (US)
Pages (from-to)141-148
Number of pages8
JournalMethods in Cell Science
Volume21
Issue number2-3
DOIs
Publication statusPublished - Aug 25 1999

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Keywords

  • Carrot cell suspension
  • Cell cycle study
  • Glyoxalase I activity
  • Inhibitors
  • Synchronization

ASJC Scopus subject areas

  • Cell Biology

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