Establishment of an Immortalized Mouse Bmp2 Knockout Dental Papilla Mesenchymal Cell Line

Wen'an Xu, Shuo Chen

Research output: Contribution to journalArticlepeer-review

Abstract

Bone morphogenetic protein 2 (Bmp2) is essential for dentin formation. Bmp2 cKO mice exhibited similar phenotype to dentinogenesis imperfecta (DGI), showing dental pulp exposure, hypomineralized dentin, and delayed odontoblast differentiation. As it is relatively difficult to obtain primary Bmp2 cKO dental papilla mesenchymal cells and to maintain a long-term culture of these primary cells, availability of immortalized deleted Bmp2 dental papilla mesenchymal cells is critical for studying the underlying mechanism of Bmp2 signal in odontogenesis. Here we describe the generation of an immortalized deleted Bmp2 dental papilla mesenchymal (iBmp2ko/ko-dp) cell line by introducing Cre fluorescent protein (GFP) into the immortalized mouse floxed Bmp2 dental papilla mesenchymal (iBmp2flox/flox-dp) cells.

Original languageEnglish (US)
Pages (from-to)13-19
Number of pages7
JournalMethods in molecular biology (Clifton, N.J.)
Volume1922
DOIs
StatePublished - Jan 1 2019

Keywords

  • Bmp2 floxed mice
  • Bone morphogenetic protein 2
  • Dental papilla mesenchymal cell line
  • Dentin formation
  • Dentinogenesis imperfecta
  • Knockout

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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