Establishment of an immortalized mouse Bmp2 knockout dental papilla mesenchymal cell line

Wen’an Xu, Shuo Chen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Bone morphogenetic protein 2 (Bmp2) is essential for dentin formation. Bmp2 cKO mice exhibited similar phenotype to dentinogenesis imperfecta (DGI), showing dental pulp exposure, hypomineralized dentin, and delayed odontoblast differentiation. As it is relatively difficult to obtain primary Bmp2 cKO dental papilla mesenchymal cells and to maintain a long-term culture of these primary cells, availability of immortalized deleted Bmp2 dental papilla mesenchymal cells is critical for studying the underlying mechanism of Bmp2 signal in odontogenesis. Here we describe the generation of an immortalized deleted Bmp2 dental papilla mesenchymal (iBmp2ko/ko-dp) cell line by introducing Cre fluorescent protein (GFP) into the immortalized mouse floxed Bmp2 dental papilla mesenchymal (iBmp2flox/flox-dp) cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages13-19
Number of pages7
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1922
ISSN (Print)1064-3745

Keywords

  • Bmp2 floxed mice
  • Bone morphogenetic protein 2
  • Dental papilla mesenchymal cell line
  • Dentin formation
  • Dentinogenesis imperfecta
  • Knockout

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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