Establishment and characterization of a new mantle cell lymphoma cell line, Mino

R. Lai, T. J. McDonnell, S. L. O'Connor, L. J. Medeiros, R. Oudat, M. Keating, M. B. Morgan, T. J. Curiel, R. J. Ford

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Mantle cell lymphoma (MCL) is a distinct type of B-cell non-Hodgkin's lymphoma characterized by cyclin D1 overexpression and the cytogenetic abnormality, the t(11;14)(q13;q32). MCL cell lines have been difficult to establish and in vitro studies of these neoplasms are scarce. We describe the establishment and characteristics of a new MCL cell line, Mino. The cells are large, growing singly and in small clumps in vitro. By flow cytometry, the immunophenotype was compatible with MCL (i.e. CD5+CD20+CD23-FMC7+). Conventional cytogenetics showed hyperdiploidy with multiple complex karyotypic abnormalities, but no evidence of the t(11;14), proven to be present only by fluorescence in situ hybridization and polymerase chain reaction (PCR) methods. Western blots showed expression of cyclin D1 but no detectable cyclin D2 and cyclin D3; the retinoblastoma protein was predominantly phosphorylated. There was expression of tumor suppressor gene products including p53, p16INK4a, and p21WAF1. Sequencing of the TP53 gene revealed a mutation (codon 147valine→glycine) in exon 5. Epstein Barr virus was absent. In summary, Mino is a new MCL cell line that may be useful to study the pathogenesis of MCL.

Original languageEnglish (US)
Pages (from-to)849-855
Number of pages7
JournalLeukemia Research
Volume26
Issue number9
DOIs
StatePublished - Jul 27 2002
Externally publishedYes

Keywords

  • Cell line
  • Cyclin D1
  • Mantle cell lymphoma
  • Mino
  • p53

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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