Establishing genomic tools and resources for Guizotia abyssinica (L.f.) Cass.-the development of a library of expressed sequence tags, microsatellite loci, and the sequencing of its chloroplast genome

Hannes Dempewolf, Nolan C. Kane, Katherine L. Ostevik, Mulatu Geleta, Michael S. Barker, Zhao Lai, Megan L. Stewart, Endashaw Bekele, Johannes M.M. Engels, Quentin C.B. Cronk, Loren H. Rieseberg

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

We present an EST library, chloroplast genome sequence, and nuclear microsatellite markers that were developed for the semi-domesticated oilseed crop noug (Guizotia abyssinica) from Ethiopia. The EST library consists of 25-711 Sanger reads, assembled into 17-538 contigs and singletons, of which 4781 were functionally annotated using the Arabidopsis Information Resource (TAIR). The age distribution of duplicated genes in the EST library shows evidence of two paleopolyploidizations-a pattern that noug shares with several other species in the Heliantheae tribe (Compositae family). From the EST library, we selected 43 microsatellites and then designed and tested primers for their amplification. The number of microsatellite alleles varied between 2 and 10 (average 4.67), and the average observed and expected heterozygosities were 0.49 and 0.54, respectively. The chloroplast genome was sequenced de novo using Illumina's sequencing technology and completed with traditional Sanger sequencing. No large re-arrangements were found between the noug and sunflower chloroplast genomes, but 1.4% of sites have indels and 1.8% show sequence divergence between the two species. We identified 34 tRNAs, 4 rRNA sequences, and 80 coding sequences, including one region (trnH-psbA) with 15% sequence divergence between noug and sunflower that may be particularly useful for phylogeographic studies in noug and its wild relatives.

Original languageEnglish (US)
Pages (from-to)1048-1058
Number of pages11
JournalMolecular Ecology Resources
Volume10
Issue number6
DOIs
StatePublished - Nov 1 2010
Externally publishedYes

Keywords

  • Chloroplast genome
  • EST library
  • Genetic resources
  • Guizotia abyssinica
  • Microsatellites
  • Paleopolyploidy

ASJC Scopus subject areas

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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