Epitopes of the G1 glycoprotein of La Crosse virus form overlapping clusters within a single antigenic site

J. A. Najjar, J. R. Gentsch, N. Nathanson, F. Gonzalez-Scarano

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Antigenic sites on the G1 glycoprotein of La Crosse bunyavirus were defined by constructing a panel of neutralizing and nonneutralizing monoclonal antibodies (F. Gonzalez-Scarano, R. E. Shope, C. H. Calisher, and N. Nathanson (1982), Virology120, 42-53). To analyze the relationship between the individual epitopes delineated by monoclonal antibodies, 11 neutralizing antibodies were used to select variant viruses. These variant viruses were tested against the panel of anti-G1 protein monoclonal antibodies by neutralization and by ELISA. The neutralization tests assigned the 11 epitopes to five groups, consisting of 6, 2, 1, 1, and 1 epitopes. ELISA tests gave a similar pattern, but also demonstrated interrelationships between four of the five epitope groups, suggesting that there may be a single immunodominant antigenic site on the G1 protein. When eight nonneutralizing anti-G1 monoclonal antibodies were tested in ELISA, they fell into three of the five epitope groups defined by neutralization; there was no evidence of a separate nonneutralizing antigenic site on the G1 protein.

Original languageEnglish (US)
Pages (from-to)426-432
Number of pages7
JournalVirology
Volume144
Issue number2
DOIs
StatePublished - Jul 30 1985

ASJC Scopus subject areas

  • Virology

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