Epigenetic reprogramming of mesenchymal stem cells

Yu Wei Leu, Tim H.M. Huang, Shu Huei Hsiao

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Scopus citations

Abstract

Mesenchymal stem cells (MSCs) are multipotent stem cells of mesodermal origin that can be isolated from various sources and induced into different cell types. Although MSCs possess immune privilege and are more easily obtained than embryonic stem cells, their propensity to tumorigenesis has not been fully explored. Epigenomic changes in DNA methylation and chromatin structure have been hypothesized to be critical in the determination of lineage-specific differentiation and tumorigenesis of MSCs, but this has not been formally proven. We applied a targeted DNA methylation method to methylate a Polycomb group protein-governed gene, Trip10, in MSCs, which accelerated the cell fate determination of MSCs. In addition, targeted methylation of HIC1 and RassF1A, both tumor suppressor genes, transformed MSCs into tumor stem cell-like cells. This new method will allow better control of the differentiation of MSCs and their use in downstream applications.

Original languageEnglish (US)
Title of host publicationEpigenetic Alterations in Oncogenesis
EditorsAdam Karpf
Pages195-211
Number of pages17
DOIs
StatePublished - Jan 1 2013

Publication series

NameAdvances in Experimental Medicine and Biology
Volume754
ISSN (Print)0065-2598

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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