Abstract
Available treatment strategies against estrogen receptor (ER)-negative breast cancer patients are limited due to their poor response to hormonal therapy. We have shown previously that the combinations of green tea polyphenols (GTPs), a dietary DNA methyltransferase inhibitor, and sulforaphane (SFN), a dietary histone deacetylase inhibitor, reactivate ERα expression in ERα-negative MDA-MB-231 cells. Here, we investigated the functional significance of ERα reactivation in the reactivation of silenced tumor suppressor genes (TSGs) in ERα-negative human breast cancer cells. We found that the treatment of MDA-MB-231 cells with the combinations of GTPs and SFN leads to the reactivation of silenced TSGs such as p21CIP1/WAF1 and KLOTHO through active chromatin modifications. Further, GTPs- and SFN-mediated reactivation of TSGs was, at least in part, dependent on ERα reactivation in ERα-negative MDA-MB-231 cells. Collectively, our findings suggest that a novel combination of bioactive dietary supplements could further be explored as an effective therapeutic option against hormonal refractory breast cancer.
Original language | English (US) |
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Pages (from-to) | 102-114 |
Number of pages | 13 |
Journal | Molecular and Cellular Endocrinology |
Volume | 406 |
DOIs | |
State | Published - May 5 2015 |
Externally published | Yes |
Keywords
- Breast cancer
- Epigenetics
- Estrogen receptor
- Green tea
- Sulforaphane
- Tumor suppressor genes
ASJC Scopus subject areas
- Endocrinology
- Molecular Biology
- Biochemistry