Epigenetic influences of low-dose bisphenol A in primary human breast epithelial cells

Yu I. Weng, Pei Yin Hsu, Sandya Liyanarachchi, Joseph Liu, Daniel E. Deatherage, Yi Wen Huang, Tao Zuo, Benjamin Rodriguez, Ching Hung Lin, Ann Lii Cheng, Hui-ming Huang

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Substantial evidence indicates that exposure to bisphenol A (BPA) during early development may increase breast cancer risk later in life. The changes may persist into puberty and adulthood, suggesting an epigenetic process being imposed in differentiated breast epithelial cells. The molecular mechanisms by which early memory of BPA exposure is imprinted in breast progenitor cells and then passed onto their epithelial progeny are not well understood. The aim of this study was to examine epigenetic changes in breast epithelial cells treated with low-dose BPA. We also investigated the effect of BPA on the ERα signaling pathway and global gene expression profiles. Compared to control cells, nuclear internalization of ERα was observed in epithelial cells preexposed to BPA. We identified 170 genes with similar expression changes in response to BPA. Functional analysis confirms that gene suppression was mediated in part through an ERα-dependent pathway. As a result of exposure to BPA or other estrogen-like chemicals, the expression of lysosomal-associated membrane protein 3 (LAMP3) became epigenetically silenced in breast epithelial cells. Furthermore, increased DNA methylation in the LAMP3 CpG island was this repressive mark preferentially occurred in ERα-positive breast tumors. These results suggest that the in vitro system developed in our laboratory is a valuable tool for exposure studies of BPA and other xenoestrogens in human cells. Individual and geographical differences may contribute to altered patterns of gene expression and DNA methylation in susceptible loci. Combination of our exposure model with epigenetic analysis and other biochemical assays can give insight into the heritable effect of low-dose BPA in human cells.

Original languageEnglish (US)
Pages (from-to)111-121
Number of pages11
JournalToxicology and Applied Pharmacology
Volume248
Issue number2
DOIs
StatePublished - Oct 2010
Externally publishedYes

Fingerprint

Epigenomics
Breast
Epithelial Cells
Lysosomal-Associated Membrane Protein 3
DNA Methylation
Gene expression
Genes
Cells
Genetic Epigenesis
Breast Neoplasms
bisphenol A
Functional analysis
CpG Islands
Puberty
Transcriptome
Individuality
Tumors
Assays
Estrogens
Stem Cells

Keywords

  • Bisphenol A
  • Breast cancer
  • DNA methylation
  • Epigenetics
  • Estrogen

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology
  • Medicine(all)

Cite this

Epigenetic influences of low-dose bisphenol A in primary human breast epithelial cells. / Weng, Yu I.; Hsu, Pei Yin; Liyanarachchi, Sandya; Liu, Joseph; Deatherage, Daniel E.; Huang, Yi Wen; Zuo, Tao; Rodriguez, Benjamin; Lin, Ching Hung; Cheng, Ann Lii; Huang, Hui-ming.

In: Toxicology and Applied Pharmacology, Vol. 248, No. 2, 10.2010, p. 111-121.

Research output: Contribution to journalArticle

Weng, YI, Hsu, PY, Liyanarachchi, S, Liu, J, Deatherage, DE, Huang, YW, Zuo, T, Rodriguez, B, Lin, CH, Cheng, AL & Huang, H 2010, 'Epigenetic influences of low-dose bisphenol A in primary human breast epithelial cells', Toxicology and Applied Pharmacology, vol. 248, no. 2, pp. 111-121. https://doi.org/10.1016/j.taap.2010.07.014
Weng, Yu I. ; Hsu, Pei Yin ; Liyanarachchi, Sandya ; Liu, Joseph ; Deatherage, Daniel E. ; Huang, Yi Wen ; Zuo, Tao ; Rodriguez, Benjamin ; Lin, Ching Hung ; Cheng, Ann Lii ; Huang, Hui-ming. / Epigenetic influences of low-dose bisphenol A in primary human breast epithelial cells. In: Toxicology and Applied Pharmacology. 2010 ; Vol. 248, No. 2. pp. 111-121.
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