Epidermal growth factor inhibits Na-P(i) cotransport in weaned and suckling rats

Mazen Arar, Hubert K. Zajicek, Ihsan Elshihabi, Moshe Levi

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

In the present study, we determined the effect of epidermal growth factor (EGF; 10 μg/100 g body wt) on sodium gradient-dependent phosphate transport (Na-P(i) cotransport) regulation in suckling (12-day-old) and weaned (24-day-old) rats. Weaned rats had higher proximal tubular brush border membrane vesicle (BBMV) Na-P(i) cotransport activity (232 ± 16 in weaned vs. 130 ± 9 pmol · 10 s-1 · mg protein-1 in suckling rats, P < 0.05). Chronic treatment with EGF induced inhibition of BBMV Na-P(i) cotransport in both suckling (130 ± 9 vs. 104 ± 7 pmol · 10 s-1 · mg protein-1, P < 0.05) and weaned rats (232 ± 16 vs. 145 ± 9 pmol · 10 s- 1 · mg protein-1, P < 0.005). The inhibitory effect was selective for Na- P(i) cotransport as there was no inhibition of Na-glucose cotransport. Weaned rats had a higher abundance of BBMV NaPi-2 protein than suckling rats (increase of 54%, P < 0.001) and a twofold increase in NaPi-2 mRNA. The EGF- induced inhibition of Na-P(i) transport was paralleled by decreases in NaPi- 2 protein abundance in both weaned (decrease of 26%, P < 0.01) and suckling (decrease of 27%, P < 0.01) animals. In contrast, there were no changes in NaPi-2 mRNA abundance. We conclude that proximal tubule BBMV Na-P(i) cotransport activity, NaPi-2 protein abundance, and NaPi-2 mRNA abundance are higher in weaned than in suckling rats. EGF inhibits Na-P(i) cotransport activity in BBMV isolated from suckling and weaned rats, and this inhibition is mediated via a decrease in NaPi-2 protein abundance, in the absence of a change in NaPi-2 mRNA.

Original languageEnglish (US)
Pages (from-to)F72-F78
JournalAmerican Journal of Physiology - Renal Physiology
Volume276
Issue number1 45-1
DOIs
StatePublished - Jan 1999

Keywords

  • NaPi-2 mRNA
  • NaPi-2 protein
  • Phosphate transporter maturation

ASJC Scopus subject areas

  • Physiology
  • Urology

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