Env-specific IgA from viremic HIV-infected subjects compromises antibodydependent cellular cytotoxicity

María Julia Ruiz, Yanina Ghiglione, Juliana Falivene, Natalia Laufer, María Pía Holgado, María Eugenia Socías, Pedro Cahn, Omar Sued, Luis Giavedoni, Horacio Salomón, María Magdalena Gherardi, Ana María Rodríguez, Gabriela Turk

    Research output: Contribution to journalArticlepeer-review

    19 Scopus citations

    Abstract

    Elucidating the factors that modulate HIV-specific antibody-dependent cellular cytotoxicity (ADCC) will help in understanding its role in HIV immunity. The aim of this study was to determine whether IgA could modify the magnitude of ADCC in HIV infection, abrogating its protective role. Plasma samples from 20 HIV-positive (HIV+) subjects enrolled during primary HIV infection (PHI), 10 chronically infected subjects (chronic), and 7 elite controllers (EC) were used. ADCC was determined by using a fluorometric ADCC assay, before and after removal of plasma IgA. Data were analyzed by using nonparametric statistics. ADCC was documented in 80% of PHI enrollment samples and in 100% of PHI 12-month, chronic, and EC samples; it peaked after acute infection, reached a plateau in chronic infection, and decreased after initiation of antiretroviral treatment (ART). Significant associations between ADCC and disease progression were found only after removal of plasma IgA from 12-month PHI samples: The magnitude of ADCC not only increased after IgA removal but also correlated with CD4+ T-cell preservation. This work provides evidence that gp120-specific IgA was capable of modifying ADCC responses during natural HIV infection for the first time and adds to similar evidence provided in other settings. Furthermore, it underscores the complexity of the ADCC phenomenon and will help in an understanding of its underlying mechanisms.

    Original languageEnglish (US)
    Pages (from-to)670-681
    Number of pages12
    JournalJournal of virology
    Volume90
    Issue number2
    DOIs
    StatePublished - 2016

    ASJC Scopus subject areas

    • Microbiology
    • Immunology
    • Insect Science
    • Virology

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