Enterocyte mitochondrial dysfunction due to oxidative stress

R. Anup; M. Madesh; K. A. Balasubramanian

Enterocyte mitochondrial dysfunction due to oxidative stress

R. Anup, M. Madesh, K. A. Balasubramanian

Research output: Contribution to journal › Article › peer-review

Abstract

The endogenous production of H₂O₂ in isolated rat intestinal mitochondria and oxidant induced damage to mitochondria were examined. There was an appreciable amount of H₂O₂ production in presence of succinate, glutamate and pyruvate, while the presence of rotenone with succinate further increased production. Superoxide generated by the X-XO system induced membrane permeability transition (MPT), calcium influx, lipid peroxidation and changes in membrane fluidity in mitochondria. A decreased mitochondrial ATPase activity and uncoupling of respiration was also observed. Spermine inhibited swelling induced by X-XO and also blocked the calcium influx and reversed the membrane fluidity changes.

Original language	English (US)
Pages (from-to)	266-271
Number of pages	6
Journal	Indian Journal of Biochemistry and Biophysics
Volume	36
Issue number	4
State	Published - Aug 1999
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry

Cite this

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title = "Enterocyte mitochondrial dysfunction due to oxidative stress",

abstract = "The endogenous production of H2O2 in isolated rat intestinal mitochondria and oxidant induced damage to mitochondria were examined. There was an appreciable amount of H2O2 production in presence of succinate, glutamate and pyruvate, while the presence of rotenone with succinate further increased production. Superoxide generated by the X-XO system induced membrane permeability transition (MPT), calcium influx, lipid peroxidation and changes in membrane fluidity in mitochondria. A decreased mitochondrial ATPase activity and uncoupling of respiration was also observed. Spermine inhibited swelling induced by X-XO and also blocked the calcium influx and reversed the membrane fluidity changes.",

author = "R. Anup and M. Madesh and Balasubramanian, {K. A.}",

year = "1999",

month = aug,

language = "English (US)",

volume = "36",

pages = "266--271",

journal = "Indian Journal of Biochemistry and Biophysics",

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publisher = "National Institute of Science Communication and Policy Research",

number = "4",

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T1 - Enterocyte mitochondrial dysfunction due to oxidative stress

AU - Anup, R.

AU - Madesh, M.

AU - Balasubramanian, K. A.

PY - 1999/8

Y1 - 1999/8

N2 - The endogenous production of H2O2 in isolated rat intestinal mitochondria and oxidant induced damage to mitochondria were examined. There was an appreciable amount of H2O2 production in presence of succinate, glutamate and pyruvate, while the presence of rotenone with succinate further increased production. Superoxide generated by the X-XO system induced membrane permeability transition (MPT), calcium influx, lipid peroxidation and changes in membrane fluidity in mitochondria. A decreased mitochondrial ATPase activity and uncoupling of respiration was also observed. Spermine inhibited swelling induced by X-XO and also blocked the calcium influx and reversed the membrane fluidity changes.

AB - The endogenous production of H2O2 in isolated rat intestinal mitochondria and oxidant induced damage to mitochondria were examined. There was an appreciable amount of H2O2 production in presence of succinate, glutamate and pyruvate, while the presence of rotenone with succinate further increased production. Superoxide generated by the X-XO system induced membrane permeability transition (MPT), calcium influx, lipid peroxidation and changes in membrane fluidity in mitochondria. A decreased mitochondrial ATPase activity and uncoupling of respiration was also observed. Spermine inhibited swelling induced by X-XO and also blocked the calcium influx and reversed the membrane fluidity changes.

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M3 - Article

C2 - 10650729

AN - SCOPUS:0033170065

SN - 0301-1208

VL - 36

SP - 266

EP - 271

JO - Indian Journal of Biochemistry and Biophysics

JF - Indian Journal of Biochemistry and Biophysics

IS - 4

ER -

Enterocyte mitochondrial dysfunction due to oxidative stress

Abstract

ASJC Scopus subject areas

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