Enhancement of rhodanese activity during controlled digestion with trypsin

Bernard L. Trumpower, Aspandier Katki, Paul Horowitz

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

When the enzyme rhodanese (EC 2. 8. 1. 1) is digested with trypsin under controlled conditions, the parent protein is converted from a polypeptide of molecular weight 32,600 to a polypeptide of molecular weight 28,800. This proteolytic conversion occurs with no loss of rhodanese activity. In fact, preliminary results indicate that the polypeptide produced by proteolysis has higher sulfur transferase activity than the native rhodanese.

Original languageEnglish (US)
Pages (from-to)532-538
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume57
Issue number2
DOIs
StatePublished - Mar 25 1974
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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