Enhancement of pathogen detection and therapy with laser-activated, functionalized gold nanoparticles

Randolph D. Glickman, Nancy J. Millenbaugh, Dickson Kirui, Saher Maswadi, Robert J. Thomas, Chad A. Oian

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Gold nanoparticles (NPs) were functionalized in order to target specific antigens associated with pathogenic viruses and bacteria, and enhance the selective diagnosis and killing of infectious agents. Gold NPs, conjugated to antibodies against human immunodeficiency virus, human T-lymphotropic virus, and hepatitis B viral proteins, were able to detect as little as 10. pg/ml of viral antigen, with excellent specificity and no cross-reactivity between antigens, using NanoLISA, a modified immunoaffinity assay. For antimicrobial use, gold NPs were conjugated to antibodies specific to Staphylococcus aureus surface proteins, incubated with suspensions of methicillin-resistant (MRSA) and -sensitive (MSSA) S. aureus, and then exposed to YAG laser. In the MRSA and MSSA populations, bacterial survival was reduced to 58% and 31% of control, respectively. Non-functionalized NPs had a negligible bactericidal effect. Dual-use, diagnostic and therapeutic functionalized gold NPs prospectively can be used alone or incorporated into existing clinical procedures.

Original languageEnglish (US)
Title of host publicationNanobiomaterials in Antimicrobial Therapy
Subtitle of host publicationApplications of Nanobiomaterials
PublisherElsevier Inc.
Pages229-260
Number of pages32
ISBN (Electronic)9780323428873
ISBN (Print)9780323428644
DOIs
StatePublished - Apr 6 2016

Keywords

  • Bacteria
  • Diagnostic
  • Laser
  • NanoLISA
  • Nanoparticles
  • Optoacoustic
  • Therapeutic
  • Virus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)

Fingerprint Dive into the research topics of 'Enhancement of pathogen detection and therapy with laser-activated, functionalized gold nanoparticles'. Together they form a unique fingerprint.

Cite this