Enhancement of pathogen detection and therapy with laser-activated, functionalized gold nanoparticles

Randolph D. Glickman; Nancy J. Millenbaugh; Dickson Kirui; Saher Maswadi; Robert J. Thomas; Chad A. Oian

doi:10.1016/B978-0-323-42864-4.00007-5

Enhancement of pathogen detection and therapy with laser-activated, functionalized gold nanoparticles

Randolph D. Glickman, Nancy J. Millenbaugh, Dickson Kirui, Saher Maswadi, Robert J. Thomas, Chad A. Oian

Ophthalmology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Gold nanoparticles (NPs) were functionalized in order to target specific antigens associated with pathogenic viruses and bacteria, and enhance the selective diagnosis and killing of infectious agents. Gold NPs, conjugated to antibodies against human immunodeficiency virus, human T-lymphotropic virus, and hepatitis B viral proteins, were able to detect as little as 10. pg/ml of viral antigen, with excellent specificity and no cross-reactivity between antigens, using NanoLISA, a modified immunoaffinity assay. For antimicrobial use, gold NPs were conjugated to antibodies specific to Staphylococcus aureus surface proteins, incubated with suspensions of methicillin-resistant (MRSA) and -sensitive (MSSA) S. aureus, and then exposed to YAG laser. In the MRSA and MSSA populations, bacterial survival was reduced to 58% and 31% of control, respectively. Non-functionalized NPs had a negligible bactericidal effect. Dual-use, diagnostic and therapeutic functionalized gold NPs prospectively can be used alone or incorporated into existing clinical procedures.

Original language	English (US)
Title of host publication	Nanobiomaterials in Antimicrobial Therapy
Subtitle of host publication	Applications of Nanobiomaterials
Publisher	Elsevier Inc.
Pages	229-260
Number of pages	32
ISBN (Electronic)	9780323428873
ISBN (Print)	9780323428644
DOIs	https://doi.org/10.1016/B978-0-323-42864-4.00007-5
State	Published - Apr 6 2016

Keywords

Bacteria
Diagnostic
Laser
NanoLISA
Nanoparticles
Optoacoustic
Therapeutic
Virus

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Engineering(all)

Access to Document

10.1016/B978-0-323-42864-4.00007-5

Fingerprint Dive into the research topics of 'Enhancement of pathogen detection and therapy with laser-activated, functionalized gold nanoparticles'. Together they form a unique fingerprint.

Cite this

Enhancement of pathogen detection and therapy with laser-activated, functionalized gold nanoparticles. / Glickman, Randolph D.; Millenbaugh, Nancy J.; Kirui, Dickson; Maswadi, Saher; Thomas, Robert J.; Oian, Chad A.

Nanobiomaterials in Antimicrobial Therapy: Applications of Nanobiomaterials. Elsevier Inc., 2016. p. 229-260.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

@inbook{e6a4115dca1c476d8aa35309182b0f4a,

title = "Enhancement of pathogen detection and therapy with laser-activated, functionalized gold nanoparticles",

abstract = "Gold nanoparticles (NPs) were functionalized in order to target specific antigens associated with pathogenic viruses and bacteria, and enhance the selective diagnosis and killing of infectious agents. Gold NPs, conjugated to antibodies against human immunodeficiency virus, human T-lymphotropic virus, and hepatitis B viral proteins, were able to detect as little as 10. pg/ml of viral antigen, with excellent specificity and no cross-reactivity between antigens, using NanoLISA, a modified immunoaffinity assay. For antimicrobial use, gold NPs were conjugated to antibodies specific to Staphylococcus aureus surface proteins, incubated with suspensions of methicillin-resistant (MRSA) and -sensitive (MSSA) S. aureus, and then exposed to YAG laser. In the MRSA and MSSA populations, bacterial survival was reduced to 58% and 31% of control, respectively. Non-functionalized NPs had a negligible bactericidal effect. Dual-use, diagnostic and therapeutic functionalized gold NPs prospectively can be used alone or incorporated into existing clinical procedures.",

keywords = "Bacteria, Diagnostic, Laser, NanoLISA, Nanoparticles, Optoacoustic, Therapeutic, Virus",

author = "Glickman, {Randolph D.} and Millenbaugh, {Nancy J.} and Dickson Kirui and Saher Maswadi and Thomas, {Robert J.} and Oian, {Chad A.}",

year = "2016",

month = apr,

day = "6",

doi = "10.1016/B978-0-323-42864-4.00007-5",

language = "English (US)",

isbn = "9780323428644",

pages = "229--260",

booktitle = "Nanobiomaterials in Antimicrobial Therapy",

publisher = "Elsevier Inc.",

}

TY - CHAP

T1 - Enhancement of pathogen detection and therapy with laser-activated, functionalized gold nanoparticles

AU - Glickman, Randolph D.

AU - Millenbaugh, Nancy J.

AU - Kirui, Dickson

AU - Maswadi, Saher

AU - Thomas, Robert J.

AU - Oian, Chad A.

PY - 2016/4/6

Y1 - 2016/4/6

N2 - Gold nanoparticles (NPs) were functionalized in order to target specific antigens associated with pathogenic viruses and bacteria, and enhance the selective diagnosis and killing of infectious agents. Gold NPs, conjugated to antibodies against human immunodeficiency virus, human T-lymphotropic virus, and hepatitis B viral proteins, were able to detect as little as 10. pg/ml of viral antigen, with excellent specificity and no cross-reactivity between antigens, using NanoLISA, a modified immunoaffinity assay. For antimicrobial use, gold NPs were conjugated to antibodies specific to Staphylococcus aureus surface proteins, incubated with suspensions of methicillin-resistant (MRSA) and -sensitive (MSSA) S. aureus, and then exposed to YAG laser. In the MRSA and MSSA populations, bacterial survival was reduced to 58% and 31% of control, respectively. Non-functionalized NPs had a negligible bactericidal effect. Dual-use, diagnostic and therapeutic functionalized gold NPs prospectively can be used alone or incorporated into existing clinical procedures.

AB - Gold nanoparticles (NPs) were functionalized in order to target specific antigens associated with pathogenic viruses and bacteria, and enhance the selective diagnosis and killing of infectious agents. Gold NPs, conjugated to antibodies against human immunodeficiency virus, human T-lymphotropic virus, and hepatitis B viral proteins, were able to detect as little as 10. pg/ml of viral antigen, with excellent specificity and no cross-reactivity between antigens, using NanoLISA, a modified immunoaffinity assay. For antimicrobial use, gold NPs were conjugated to antibodies specific to Staphylococcus aureus surface proteins, incubated with suspensions of methicillin-resistant (MRSA) and -sensitive (MSSA) S. aureus, and then exposed to YAG laser. In the MRSA and MSSA populations, bacterial survival was reduced to 58% and 31% of control, respectively. Non-functionalized NPs had a negligible bactericidal effect. Dual-use, diagnostic and therapeutic functionalized gold NPs prospectively can be used alone or incorporated into existing clinical procedures.

KW - Bacteria

KW - Diagnostic

KW - Laser

KW - NanoLISA

KW - Nanoparticles

KW - Optoacoustic

KW - Therapeutic

KW - Virus

UR - http://www.scopus.com/inward/record.url?scp=84969150908&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84969150908&partnerID=8YFLogxK

U2 - 10.1016/B978-0-323-42864-4.00007-5

DO - 10.1016/B978-0-323-42864-4.00007-5

M3 - Chapter

AN - SCOPUS:84969150908

SN - 9780323428644

SP - 229

EP - 260

BT - Nanobiomaterials in Antimicrobial Therapy

PB - Elsevier Inc.

ER -