Enhancement of pathogen detection and therapy with laser-activated, functionalized gold nanoparticles

Randolph D. Glickman; Nancy J. Millenbaugh; Dickson Kirui; Saher Maswadi; Robert J. Thomas; Chad A. Oian

doi:10.1016/B978-0-323-42864-4.00007-5

Enhancement of pathogen detection and therapy with laser-activated, functionalized gold nanoparticles

Randolph D. Glickman, Nancy J. Millenbaugh, Dickson Kirui, Saher Maswadi, Robert J. Thomas, Chad A. Oian

Ophthalmology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Gold nanoparticles (NPs) were functionalized in order to target specific antigens associated with pathogenic viruses and bacteria, and enhance the selective diagnosis and killing of infectious agents. Gold NPs, conjugated to antibodies against human immunodeficiency virus, human T-lymphotropic virus, and hepatitis B viral proteins, were able to detect as little as 10. pg/ml of viral antigen, with excellent specificity and no cross-reactivity between antigens, using NanoLISA, a modified immunoaffinity assay. For antimicrobial use, gold NPs were conjugated to antibodies specific to Staphylococcus aureus surface proteins, incubated with suspensions of methicillin-resistant (MRSA) and -sensitive (MSSA) S. aureus, and then exposed to YAG laser. In the MRSA and MSSA populations, bacterial survival was reduced to 58% and 31% of control, respectively. Non-functionalized NPs had a negligible bactericidal effect. Dual-use, diagnostic and therapeutic functionalized gold NPs prospectively can be used alone or incorporated into existing clinical procedures.

Original language	English (US)
Title of host publication	Nanobiomaterials in Antimicrobial Therapy
Subtitle of host publication	Applications of Nanobiomaterials
Publisher	Elsevier Inc.
Pages	229-260
Number of pages	32
ISBN (Electronic)	9780323428873
ISBN (Print)	9780323428644
DOIs	https://doi.org/10.1016/B978-0-323-42864-4.00007-5
State	Published - Apr 6 2016

Keywords

Bacteria
Diagnostic
Laser
NanoLISA
Nanoparticles
Optoacoustic
Therapeutic
Virus

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Engineering(all)

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Enhancement of pathogen detection and therapy with laser-activated, functionalized gold nanoparticles. / Glickman, Randolph D.; Millenbaugh, Nancy J.; Kirui, Dickson; Maswadi, Saher; Thomas, Robert J.; Oian, Chad A.

Nanobiomaterials in Antimicrobial Therapy: Applications of Nanobiomaterials. Elsevier Inc., 2016. p. 229-260.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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abstract = "Gold nanoparticles (NPs) were functionalized in order to target specific antigens associated with pathogenic viruses and bacteria, and enhance the selective diagnosis and killing of infectious agents. Gold NPs, conjugated to antibodies against human immunodeficiency virus, human T-lymphotropic virus, and hepatitis B viral proteins, were able to detect as little as 10. pg/ml of viral antigen, with excellent specificity and no cross-reactivity between antigens, using NanoLISA, a modified immunoaffinity assay. For antimicrobial use, gold NPs were conjugated to antibodies specific to Staphylococcus aureus surface proteins, incubated with suspensions of methicillin-resistant (MRSA) and -sensitive (MSSA) S. aureus, and then exposed to YAG laser. In the MRSA and MSSA populations, bacterial survival was reduced to 58% and 31% of control, respectively. Non-functionalized NPs had a negligible bactericidal effect. Dual-use, diagnostic and therapeutic functionalized gold NPs prospectively can be used alone or incorporated into existing clinical procedures.",

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note = "Funding Information: This work was supported by the Naval Medical Research Unit San Antonio work unit number G1025 and Office of Naval Research Grant No. N00014-08-1-0193 (RDG). The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, nor the US Government. NJM and DK are employees of the US Government. This work was prepared as part of their official duties. Title 17 USC §105 provides that “Copyright protection under this title is not available for any work of the United States Government.” Title 17 USC §101 defines a US Government work as a work prepared by a military service member or employee of the US Government as part of that person{\textquoteright}s official duties. Publisher Copyright: {\textcopyright} 2016 Elsevier Inc. All rights reserved. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

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N2 - Gold nanoparticles (NPs) were functionalized in order to target specific antigens associated with pathogenic viruses and bacteria, and enhance the selective diagnosis and killing of infectious agents. Gold NPs, conjugated to antibodies against human immunodeficiency virus, human T-lymphotropic virus, and hepatitis B viral proteins, were able to detect as little as 10. pg/ml of viral antigen, with excellent specificity and no cross-reactivity between antigens, using NanoLISA, a modified immunoaffinity assay. For antimicrobial use, gold NPs were conjugated to antibodies specific to Staphylococcus aureus surface proteins, incubated with suspensions of methicillin-resistant (MRSA) and -sensitive (MSSA) S. aureus, and then exposed to YAG laser. In the MRSA and MSSA populations, bacterial survival was reduced to 58% and 31% of control, respectively. Non-functionalized NPs had a negligible bactericidal effect. Dual-use, diagnostic and therapeutic functionalized gold NPs prospectively can be used alone or incorporated into existing clinical procedures.

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