Gold nanoparticles (NPs) were functionalized in order to target specific antigens associated with pathogenic viruses and bacteria, and enhance the selective diagnosis and killing of infectious agents. Gold NPs, conjugated to antibodies against human immunodeficiency virus, human T-lymphotropic virus, and hepatitis B viral proteins, were able to detect as little as 10. pg/ml of viral antigen, with excellent specificity and no cross-reactivity between antigens, using NanoLISA, a modified immunoaffinity assay. For antimicrobial use, gold NPs were conjugated to antibodies specific to Staphylococcus aureus surface proteins, incubated with suspensions of methicillin-resistant (MRSA) and -sensitive (MSSA) S. aureus, and then exposed to YAG laser. In the MRSA and MSSA populations, bacterial survival was reduced to 58% and 31% of control, respectively. Non-functionalized NPs had a negligible bactericidal effect. Dual-use, diagnostic and therapeutic functionalized gold NPs prospectively can be used alone or incorporated into existing clinical procedures.
|Original language||English (US)|
|Title of host publication||Nanobiomaterials in Antimicrobial Therapy|
|Subtitle of host publication||Applications of Nanobiomaterials|
|Number of pages||32|
|State||Published - Apr 6 2016|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)