Enhanced release of immunoreactive CGRP and substance P from spinal dorsal horn slices occurs during carrageenan inflammation

Mary G. Garry; Kenneth M. Hargreaves

doi:10.1016/0006-8993(92)90328-7

Enhanced release of immunoreactive CGRP and substance P from spinal dorsal horn slices occurs during carrageenan inflammation

Mary G. Garry
, Kenneth M. Hargreaves

Research output: Contribution to journal › Article › peer-review

127 Scopus citations

Abstract

In vitro superfusion was used to examine the effect of inflammation on the release of immunoreactive calcitonin gene-related peptide (iCGRP) and substance P(iSP) from the dorsal horn of rats. Three hours after carrageenan, hindpaws exhibited hyperalgesia, edema, and hyperthermia. Spontaneous and capsaicin-evoked release of iCGRP and iSP were significantly increased following inflammation. The enhanced release of iCGRP and iSP in the dorsal horn may serve as a biochemical marker for the development of hyperalgesia.

Original language	English (US)
Pages (from-to)	139-142
Number of pages	4
Journal	Brain Research
Volume	582
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(92)90328-7
State	Published - Jun 5 1992
Externally published	Yes

Keywords

Calcitonin gene-related peptide
Carrageenan
Dorsal horn
Inflammation
Pain
Spinal cord
Substance P

ASJC Scopus subject areas

Clinical Neurology
Molecular Biology
General Neuroscience
Developmental Biology

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10.1016/0006-8993(92)90328-7

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title = "Enhanced release of immunoreactive CGRP and substance P from spinal dorsal horn slices occurs during carrageenan inflammation",

abstract = "In vitro superfusion was used to examine the effect of inflammation on the release of immunoreactive calcitonin gene-related peptide (iCGRP) and substance P(iSP) from the dorsal horn of rats. Three hours after carrageenan, hindpaws exhibited hyperalgesia, edema, and hyperthermia. Spontaneous and capsaicin-evoked release of iCGRP and iSP were significantly increased following inflammation. The enhanced release of iCGRP and iSP in the dorsal horn may serve as a biochemical marker for the development of hyperalgesia.",

keywords = "Calcitonin gene-related peptide, Carrageenan, Dorsal horn, Inflammation, Pain, Spinal cord, Substance P",

author = "Garry, \{Mary G.\} and Hargreaves, \{Kenneth M.\}",

note = "Funding Information: The authors wish to thank Dr. Michael Vasko for his generous assistance with developing the superfusion technique. This research was supported in part by NRSA Fellowship F32-DEO5606 (M.G.G.) 3 Fitzgerald, M., Capsaicin sensitive neurons. A review, Pain, 15 (1983) 145-160. 4 Hargreaves, K., Dubner, R., Brown, E, Flores, C. and Joris, J., A new and sensitive method for measuring thermal nocicep-tion in cutaneous hyperalgesia, Pain, 32 (1988) 77-78. 5 Iverfeldt, P., Serfozo, P., Diaz Arnesto, L. and Bartfai, T., Dif-ferential release of coexisting neurotransmitters: frequency de-",

year = "1992",

month = jun,

day = "5",

doi = "10.1016/0006-8993(92)90328-7",

language = "English (US)",

volume = "582",

pages = "139--142",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier BV",

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TY - JOUR

T1 - Enhanced release of immunoreactive CGRP and substance P from spinal dorsal horn slices occurs during carrageenan inflammation

AU - Garry, Mary G.

AU - Hargreaves, Kenneth M.

N1 - Funding Information: The authors wish to thank Dr. Michael Vasko for his generous assistance with developing the superfusion technique. This research was supported in part by NRSA Fellowship F32-DEO5606 (M.G.G.) 3 Fitzgerald, M., Capsaicin sensitive neurons. A review, Pain, 15 (1983) 145-160. 4 Hargreaves, K., Dubner, R., Brown, E, Flores, C. and Joris, J., A new and sensitive method for measuring thermal nocicep-tion in cutaneous hyperalgesia, Pain, 32 (1988) 77-78. 5 Iverfeldt, P., Serfozo, P., Diaz Arnesto, L. and Bartfai, T., Dif-ferential release of coexisting neurotransmitters: frequency de-

PY - 1992/6/5

Y1 - 1992/6/5

N2 - In vitro superfusion was used to examine the effect of inflammation on the release of immunoreactive calcitonin gene-related peptide (iCGRP) and substance P(iSP) from the dorsal horn of rats. Three hours after carrageenan, hindpaws exhibited hyperalgesia, edema, and hyperthermia. Spontaneous and capsaicin-evoked release of iCGRP and iSP were significantly increased following inflammation. The enhanced release of iCGRP and iSP in the dorsal horn may serve as a biochemical marker for the development of hyperalgesia.

AB - In vitro superfusion was used to examine the effect of inflammation on the release of immunoreactive calcitonin gene-related peptide (iCGRP) and substance P(iSP) from the dorsal horn of rats. Three hours after carrageenan, hindpaws exhibited hyperalgesia, edema, and hyperthermia. Spontaneous and capsaicin-evoked release of iCGRP and iSP were significantly increased following inflammation. The enhanced release of iCGRP and iSP in the dorsal horn may serve as a biochemical marker for the development of hyperalgesia.

KW - Calcitonin gene-related peptide

KW - Carrageenan

KW - Dorsal horn

KW - Inflammation

KW - Pain

KW - Spinal cord

KW - Substance P

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UR - https://www.scopus.com/pages/publications/0026637909#tab=citedBy

U2 - 10.1016/0006-8993(92)90328-7

DO - 10.1016/0006-8993(92)90328-7

M3 - Article

C2 - 1379871

AN - SCOPUS:0026637909

SN - 0006-8993

VL - 582

SP - 139

EP - 142

JO - Brain Research

JF - Brain Research

IS - 1

ER -

Enhanced release of immunoreactive CGRP and substance P from spinal dorsal horn slices occurs during carrageenan inflammation

Abstract

Keywords

ASJC Scopus subject areas

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