Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds

Lorenz Meinel, Vassilis Karageorgiou, Sandra Hofmann, Robert Fajardo, Brian Snyder, Chunmei Li, Ludwig Zichner, Robert Langer, Gordana Vunjak-Novakovic, David L. Kaplan

Research output: Contribution to journalArticle

280 Citations (Scopus)

Abstract

Porous biodegradable silk scaffolds and human bone marrow derived mesenchymal stem cells (hMSCs) were used to engineer bone-like tissue in vitro. Two different scaffolds with the same microstructure were studied: collagen (to assess the effects of fast degradation) and silk with covalently bound RGD sequences (to assess the effects of enhanced cell attachment and slow degradation). The hM-SCs were isolated, expanded in culture, characterized with respect to the expression of surface markers and ability for chondrogenic and osteogenic differentiation, seeded on scaffolds, and cultured for up to 4 weeks. Histological analysis and microcomputer tomography showed the development of up to 1.2-mm-long interconnected and organized bonelike trabeculae with cuboid cells on the silk-RGD scaffolds, features still present but to a lesser extent on silk scaffolds and absent on the collagen scaffolds. The X-ray diffraction pattern of the deposited bone corresponded to hydroxyapatite present in the native bone. Biochemical analysis showed increased mineralization on silk-RGD scaffolds compared with either silk or collagen scaffolds after 4 weeks. Expression of bone sialoprotein, osteopontin, and bone morphogenetic protein 2 was significantly higher for hMSCs cultured in osteogenic than control medium both after 2 and 4 weeks in culture. The results suggest that RGD-silk scaffolds are particularly suitable for autologous bone tissue engineering, presumably because of their stable macroporous structure, tailorable mechanical properties matching those of native bone, and slow degradation.

Original languageEnglish (US)
Pages (from-to)25-34
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume71
Issue number1
DOIs
StatePublished - Oct 1 2004
Externally publishedYes

Fingerprint

Silk
Stem cells
Scaffolds
Bone Marrow Cells
Bone
Stem Cells
Scaffolds (biology)
Tissue
Bone and Bones
Collagen
Mesenchymal Stromal Cells
Degradation
Bone Marrow
Integrin-Binding Sialoprotein
Bone Morphogenetic Protein 2
Osteopontin
Microcomputers
Durapatite
Tissue Engineering
In Vitro Techniques

Keywords

  • Hydroxyapatite
  • Osteogenic
  • Silk
  • Stem cells
  • Tissue engineering

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Meinel, L., Karageorgiou, V., Hofmann, S., Fajardo, R., Snyder, B., Li, C., ... Kaplan, D. L. (2004). Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds. Journal of Biomedical Materials Research - Part A, 71(1), 25-34. https://doi.org/10.1002/jbm.a.30117

Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds. / Meinel, Lorenz; Karageorgiou, Vassilis; Hofmann, Sandra; Fajardo, Robert; Snyder, Brian; Li, Chunmei; Zichner, Ludwig; Langer, Robert; Vunjak-Novakovic, Gordana; Kaplan, David L.

In: Journal of Biomedical Materials Research - Part A, Vol. 71, No. 1, 01.10.2004, p. 25-34.

Research output: Contribution to journalArticle

Meinel, L, Karageorgiou, V, Hofmann, S, Fajardo, R, Snyder, B, Li, C, Zichner, L, Langer, R, Vunjak-Novakovic, G & Kaplan, DL 2004, 'Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds', Journal of Biomedical Materials Research - Part A, vol. 71, no. 1, pp. 25-34. https://doi.org/10.1002/jbm.a.30117
Meinel, Lorenz ; Karageorgiou, Vassilis ; Hofmann, Sandra ; Fajardo, Robert ; Snyder, Brian ; Li, Chunmei ; Zichner, Ludwig ; Langer, Robert ; Vunjak-Novakovic, Gordana ; Kaplan, David L. / Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds. In: Journal of Biomedical Materials Research - Part A. 2004 ; Vol. 71, No. 1. pp. 25-34.
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