TY - JOUR
T1 - Endothelin-stimulated nitric oxide production in the isolated Kupffer cell
AU - Stephenson, Katherine
AU - Gupta, Anjana
AU - Mustafa, Shamimunisa B.
AU - Halff, Glenn A.
PY - 1997/12
Y1 - 1997/12
N2 - Endothelin (ET) is a potent peptide mediator exhibiting a wide variety of effects in both the parenchymal and nonparenchymal hepatic cells. In the Kupffer cell, ET activates several transmembrane signaling pathways to generate numerous second messengers including the phospholipase C-generated products inositol-1,4,5-trisphosphate and diacylglycerol and the cyclooxygenase product prostaglandin Eg via specific ET(B)-type receptors. In addition to these findings, we have now demonstrated that endothelin stimulates the production of nitric oxide (NO) in the Kupffer cell in a time- and concentration-dependent manner. Western blot analysis indicates that ET- stimulated NO production occurs though activation of the inducible form of the nitric oxide synthase enzyme. These findings have important implications as the stimulation of NO production by ET may be part of the physiological response to inflammation or infection. Elevated levels of ET and NO have been found to be associated with numerous hepatic pathophysiological conditions that may contribute to derangements in the vascular system seen in these conditions.
AB - Endothelin (ET) is a potent peptide mediator exhibiting a wide variety of effects in both the parenchymal and nonparenchymal hepatic cells. In the Kupffer cell, ET activates several transmembrane signaling pathways to generate numerous second messengers including the phospholipase C-generated products inositol-1,4,5-trisphosphate and diacylglycerol and the cyclooxygenase product prostaglandin Eg via specific ET(B)-type receptors. In addition to these findings, we have now demonstrated that endothelin stimulates the production of nitric oxide (NO) in the Kupffer cell in a time- and concentration-dependent manner. Western blot analysis indicates that ET- stimulated NO production occurs though activation of the inducible form of the nitric oxide synthase enzyme. These findings have important implications as the stimulation of NO production by ET may be part of the physiological response to inflammation or infection. Elevated levels of ET and NO have been found to be associated with numerous hepatic pathophysiological conditions that may contribute to derangements in the vascular system seen in these conditions.
KW - Endothelin
KW - Kupffer cell
KW - Nitric oxide
KW - Nitric oxide synthase
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U2 - 10.1006/jsre.1997.5191
DO - 10.1006/jsre.1997.5191
M3 - Article
C2 - 9441809
AN - SCOPUS:0031426227
SN - 0022-4804
VL - 73
SP - 149
EP - 154
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 2
ER -