Endothelial targeting of semi-permeable polymer nanocarriers for enzyme therapies

Thomas D. Dziubla, Vladimir V. Shuvaev, Nan Kang Hong, Brian J. Hawkins, Madesh Muniswamy, Hajime Takano, Eric Simone, Marian T. Nakada, Aron Fisher, Steven M. Albelda, Vladimir R. Muzykantov

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The medical utility of proteins, e.g. therapeutic enzymes, is greatly restricted by their labile nature and inadequate delivery. Most therapeutic enzymes do not accumulate in their targets and are inactivated by proteases. Targeting of enzymes encapsulated into substrate-permeable polymer nano-carriers (PNC) impermeable for proteases might overcome these limitations. To test this hypothesis, we designed endothelial targeted PNC loaded with catalase, an H2O2-detoxifying enzyme, and tested if this approach protects against vascular oxidative stress, a pathological process implicated in ischemia-reperfusion and other disease conditions. Encapsulation of catalase (MW 247 kD), peroxidase (MW 42 kD) and xanthine oxidase (XO, MW 300 kD) into ∼300 nm diameter PNC composed of co-polymers of polyethylene glycol and poly-lactic/poly-glycolic acid (PEG-PLGA) was in the range ∼10% for all enzymes. PNC/catalase and PNC/peroxidase were protected from external proteolysis and exerted enzymatic activity on their PNC diffusible substrates, H2O2 and ortho-phenylendiamine, whereas activity of encapsulated XO was negligible due to polymer impermeability to the substrate. PNC targeted to platelet-endothelial cell (EC) adhesion molecule-1 delivered active encapsulated catalase to ECs and protected the endothelium against oxidative stress in cell culture and animal studies. Vascular targeting of PNC-loaded detoxifying enzymes may find wide medical applications including management of oxidative stress and other toxicities.

Original languageEnglish (US)
Pages (from-to)215-227
Number of pages13
JournalBiomaterials
Volume29
Issue number2
DOIs
StatePublished - Jan 1 2008
Externally publishedYes

Fingerprint

Enzyme Therapy
Polymers
Enzymes
Catalase
Oxidative stress
Oxidative Stress
glycolic acid
Peroxidase
Blood Vessels
Peptide Hydrolases
Substrates
Proteolysis
CD31 Antigens
Xanthine Oxidase
Cell adhesion
Endothelial cells
Medical applications
Pathologic Processes
Platelets
Encapsulation

Keywords

  • Antioxidant
  • Drug delivery
  • Enzyme
  • Nanoparticle
  • Polylactic acid

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Dziubla, T. D., Shuvaev, V. V., Hong, N. K., Hawkins, B. J., Muniswamy, M., Takano, H., ... Muzykantov, V. R. (2008). Endothelial targeting of semi-permeable polymer nanocarriers for enzyme therapies. Biomaterials, 29(2), 215-227. https://doi.org/10.1016/j.biomaterials.2007.09.023

Endothelial targeting of semi-permeable polymer nanocarriers for enzyme therapies. / Dziubla, Thomas D.; Shuvaev, Vladimir V.; Hong, Nan Kang; Hawkins, Brian J.; Muniswamy, Madesh; Takano, Hajime; Simone, Eric; Nakada, Marian T.; Fisher, Aron; Albelda, Steven M.; Muzykantov, Vladimir R.

In: Biomaterials, Vol. 29, No. 2, 01.01.2008, p. 215-227.

Research output: Contribution to journalArticle

Dziubla, TD, Shuvaev, VV, Hong, NK, Hawkins, BJ, Muniswamy, M, Takano, H, Simone, E, Nakada, MT, Fisher, A, Albelda, SM & Muzykantov, VR 2008, 'Endothelial targeting of semi-permeable polymer nanocarriers for enzyme therapies', Biomaterials, vol. 29, no. 2, pp. 215-227. https://doi.org/10.1016/j.biomaterials.2007.09.023
Dziubla, Thomas D. ; Shuvaev, Vladimir V. ; Hong, Nan Kang ; Hawkins, Brian J. ; Muniswamy, Madesh ; Takano, Hajime ; Simone, Eric ; Nakada, Marian T. ; Fisher, Aron ; Albelda, Steven M. ; Muzykantov, Vladimir R. / Endothelial targeting of semi-permeable polymer nanocarriers for enzyme therapies. In: Biomaterials. 2008 ; Vol. 29, No. 2. pp. 215-227.
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