Endothelial nitric oxide synthase control mechanisms in the cutaneous vasculature of humans in vivo

Dean L. Kellogg, Joan L. Zhao, Yubo Wu

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

Nitric oxide (NO) participates in locally mediated vasodilation induced by increased local skin temperature (Tloc) and in sympathetically mediated vasodilation during whole body heat stress. We hypothesized that endothelial NOS (eNOS) participates in the former, but not the latter, response. We tested this hypothesis by examining the effects of the eNOS antagonist NG-amino-L-arginine (L-NAA) on skin blood flow (SkBF) responses to increased Tloc and whole body heat stress. Microdialysis probes were inserted into forearm skin for drug delivery. One microdialysis site was perfused with L-NAA in Ringer solution and a second site with Ringer solution alone. SkBF [laser-Doppler flowmetry (LDF)] and blood pressure [mean arterial pressure (MAP)] were monitored, and cutaneous vascular conductance (CVC) was calculated (CVC = LDF ÷ MAP). In protocol 1, Tloc was controlled with LDF/local heating units. Tloc initially was held at 34°C and then increased to 41.5°C. In protocol 2, after a normothermic period, whole body heat stress was induced (water-perfused suits). At the end of both protocols, 58 mM sodium nitroprusside was perfused at both microdialysis sites to cause maximal vasodilation for data normalization. In protocol 1, CVC at 34°C Tloc did not differ between L-NAA-treated and untreated sites (P > 0.05). Local skin warming to 41.5°C Tloc increased CVC at both sites. This response was attenuated at L-NAA-treated sites (P < 0.05). In protocol 2, during normothermia, CVC did not differ between L-NAA-treated and untreated sites (P > 0.05). During heat stress, CVC rose to similar levels at L-NAA-treated and untreated sites (P > 0.05). We conclude that eNOS is predominantly responsible for NO generation in skin during responses to increased Tloc, but not during reflex responses to whole body heat stress.

Original languageEnglish (US)
Pages (from-to)H123-H129
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume295
Issue number1
DOIs
StatePublished - Jul 2008

Keywords

  • Laser-Doppler flowmetry
  • Microdialysis
  • NOS III
  • Nitric oxide
  • Skin blood flow

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Endothelial nitric oxide synthase control mechanisms in the cutaneous vasculature of humans in vivo'. Together they form a unique fingerprint.

  • Cite this