TY - JOUR
T1 - Endopeptidase-24.15 in rat hypothalamic/pituitary/gonadal axis
AU - Pierotti, Adrian R.
AU - Lasdun, Abraham
AU - Ayala, Julia M.
AU - Roberts, James L.
AU - Molineaux, Christopher J.
N1 - Funding Information:
We gratefully acknowledge Dr. George Fink for helpful suggestions regarding the manuscript. Funding for this research was provided by Grants DA04218 (C.J.M.), DK39029 (J.L.R.) and DIC25377 (to our colleague Dr. Marian Orlowski, who helped us in the initial stages of this research and provided the substrates and inhibitors used in these studies).
PY - 1991/4
Y1 - 1991/4
N2 - Endopeptidase-24.15 (E.C. 3.4.24.15; EP-24.15) cleaves several substrates found in the hypothalamic/pituitary/gonadal axis, including gonadotropin-releasing hormone (GnRH) and the opioid peptides of the dynorphin family. We have examined the activity of EP-24.15 in these tissues as a function of maturation, of the estrous cycle, and in response to ovariectomy and estrogen replacement. A developmental regulation of EP-24.15-specific activity is apparent in anterior pituitary, in hypothalamus, and in the gonads. EP-24.15 is increased in the preoptic area and is decreased in the anterior pituitary in both male and female rats prior to puberty. The specific activity of EP-24.15 was increased following ovariectomy in the anterior pituitary and within medial and lateral preoptic nuclei. Testicular specific activity of EP-24.15 increased with age in a linear fashion, while ovarian EP-24.15 activity increased immediately prior to puberty, but returned to prepubertal levels by 65 days of age. The relevance of EP-24.15 to the metabolism of specific peptides is discussed.
AB - Endopeptidase-24.15 (E.C. 3.4.24.15; EP-24.15) cleaves several substrates found in the hypothalamic/pituitary/gonadal axis, including gonadotropin-releasing hormone (GnRH) and the opioid peptides of the dynorphin family. We have examined the activity of EP-24.15 in these tissues as a function of maturation, of the estrous cycle, and in response to ovariectomy and estrogen replacement. A developmental regulation of EP-24.15-specific activity is apparent in anterior pituitary, in hypothalamus, and in the gonads. EP-24.15 is increased in the preoptic area and is decreased in the anterior pituitary in both male and female rats prior to puberty. The specific activity of EP-24.15 was increased following ovariectomy in the anterior pituitary and within medial and lateral preoptic nuclei. Testicular specific activity of EP-24.15 increased with age in a linear fashion, while ovarian EP-24.15 activity increased immediately prior to puberty, but returned to prepubertal levels by 65 days of age. The relevance of EP-24.15 to the metabolism of specific peptides is discussed.
KW - Dynorphin
KW - Gonadotropin-releasing hormone
KW - Luteinizing hormone
KW - Luteinizing hormone-releasing hormone
KW - Metalloendopeptidase
UR - http://www.scopus.com/inward/record.url?scp=0026067066&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026067066&partnerID=8YFLogxK
U2 - 10.1016/0303-7207(91)90264-S
DO - 10.1016/0303-7207(91)90264-S
M3 - Article
C2 - 1820981
AN - SCOPUS:0026067066
SN - 0303-7207
VL - 76
SP - 95
EP - 103
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 1-3
ER -