Endogenous cholecystokinin reduces food intake and increases Fos-like immunoreactivity in the dorsal vagal complex but not in the myenteric plexus by CCK1 receptor in the adult rat

Cherese N. Sullivan, Shannon J. Raboin, Stephen Gulley, Ntwenzi T. Sinzobahamvya, Gary M. Green, Joseph R. Reeve, Ayman I. Sayegh

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

We hypothesized that endogenous CCK reduces food intake by activating the dorsal vagal complex (DVC) and the myenteric neurons of the gut. To test this hypothesis, adult rats were given camostat mesilate; a nonnutrient releaser of endogenous CCK, by orogastric gavage, and Fos-like immunoreactivity (Fos-LI) was quantified in the DVC and the myenteric plexus. The results for endogenous CCK were compared with those for exogenous CCK-8. Exogenous CCK-8 reduced food intake and stimulated Fos-LI in the DVC and in myenteric neurons of the duodenum and jejunum. In comparison, endogenous CCK reduced food intake and increased DVC Fos-LI but did not increase Fos-LI in the myenteric plexus. Similar to CCK-8, devazepide, a specific CCK1 receptor antagonist, and not L365,260, a specific CCK2 receptor antagonist, attenuated the reduction of food intake by camostat. In addition, Fos-LI in the DVC in response to both exogenous CCK-8 and camostat administration was significantly attenuated by vagotomy, as well as by blocking CCK1 receptors. These results demonstrate for the first time that reduction of food intake in adult rats by endogenous CCK released by a nonnutrient mechanism requires CCK 1 receptors, the vagus nerve, and activation of the DVC, but not the myenteric plexus.

Original languageEnglish (US)
Pages (from-to)R1071-R1080
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume292
Issue number3
DOIs
StatePublished - Mar 2007
Externally publishedYes

Keywords

  • Camostat
  • Devazepide
  • Vagotomy

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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