Emergence of beta adrenergic-responsive hepatic glycogenolysis in male rats during post-maturational aging

Michael S. Katz, Christopher L. McNair, Tazuko K. Hymer, Sarah R. Boland

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Adrenergic-stimulated glycogenolysis (estimated as glucose output) was determined in hepatocytes from 7, 14, 20, and 24 mo old male Fischer 344 rats. Glucose output in response to the beta adrenergic agonist isoproterenol was minimal at 7 mo but increased progressively with increasing age. At all ages the isoproterenol response was concentration dependent and was inhibited by the beta adrenergic antagonist propranolol. Stimulation of glucose output by the mixed alpha-beta agonist epinephrine also increased between 7 and 24 mo. Glycogenolytic responses to alpha agonist (assessed in the presence of epinephrine and excess beta antagonist), glucagon, and forskolin did not increase substantially with age and at 24 mo were less than the response to beta agonist. In hepatocyte homogenates adenylate cyclase activation by beta agonist but not glucagon and forskolin increased between 7 and 24 mo. These results suggest that adrenergic stimulation of glycogenolysis, which in young adult male rats is generally attributed to alpha adrenergic-mediated processes, becomes mediated predominantly by beta adrenergic-responsive adenylate cyclase during post-maturational aging.

Original languageEnglish (US)
Pages (from-to)724-730
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume147
Issue number2
DOIs
StatePublished - Sep 15 1987

Fingerprint

Glycogenolysis
Adrenergic Agents
Rats
Aging of materials
Liver
Colforsin
Glucagon
Isoproterenol
Adenylyl Cyclases
Glucose
Epinephrine
Hepatocytes
Adrenergic beta-Agonists
Adrenergic beta-Antagonists
Inbred F344 Rats
Propranolol
Young Adult
Chemical activation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Emergence of beta adrenergic-responsive hepatic glycogenolysis in male rats during post-maturational aging. / Katz, Michael S.; McNair, Christopher L.; Hymer, Tazuko K.; Boland, Sarah R.

In: Biochemical and Biophysical Research Communications, Vol. 147, No. 2, 15.09.1987, p. 724-730.

Research output: Contribution to journalArticle

Katz, Michael S. ; McNair, Christopher L. ; Hymer, Tazuko K. ; Boland, Sarah R. / Emergence of beta adrenergic-responsive hepatic glycogenolysis in male rats during post-maturational aging. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 147, No. 2. pp. 724-730.
@article{1294ffb8ef9a486ab16cf8eac0495524,
title = "Emergence of beta adrenergic-responsive hepatic glycogenolysis in male rats during post-maturational aging",
abstract = "Adrenergic-stimulated glycogenolysis (estimated as glucose output) was determined in hepatocytes from 7, 14, 20, and 24 mo old male Fischer 344 rats. Glucose output in response to the beta adrenergic agonist isoproterenol was minimal at 7 mo but increased progressively with increasing age. At all ages the isoproterenol response was concentration dependent and was inhibited by the beta adrenergic antagonist propranolol. Stimulation of glucose output by the mixed alpha-beta agonist epinephrine also increased between 7 and 24 mo. Glycogenolytic responses to alpha agonist (assessed in the presence of epinephrine and excess beta antagonist), glucagon, and forskolin did not increase substantially with age and at 24 mo were less than the response to beta agonist. In hepatocyte homogenates adenylate cyclase activation by beta agonist but not glucagon and forskolin increased between 7 and 24 mo. These results suggest that adrenergic stimulation of glycogenolysis, which in young adult male rats is generally attributed to alpha adrenergic-mediated processes, becomes mediated predominantly by beta adrenergic-responsive adenylate cyclase during post-maturational aging.",
author = "Katz, {Michael S.} and McNair, {Christopher L.} and Hymer, {Tazuko K.} and Boland, {Sarah R.}",
year = "1987",
month = "9",
day = "15",
doi = "10.1016/0006-291X(87)90990-9",
language = "English (US)",
volume = "147",
pages = "724--730",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Emergence of beta adrenergic-responsive hepatic glycogenolysis in male rats during post-maturational aging

AU - Katz, Michael S.

AU - McNair, Christopher L.

AU - Hymer, Tazuko K.

AU - Boland, Sarah R.

PY - 1987/9/15

Y1 - 1987/9/15

N2 - Adrenergic-stimulated glycogenolysis (estimated as glucose output) was determined in hepatocytes from 7, 14, 20, and 24 mo old male Fischer 344 rats. Glucose output in response to the beta adrenergic agonist isoproterenol was minimal at 7 mo but increased progressively with increasing age. At all ages the isoproterenol response was concentration dependent and was inhibited by the beta adrenergic antagonist propranolol. Stimulation of glucose output by the mixed alpha-beta agonist epinephrine also increased between 7 and 24 mo. Glycogenolytic responses to alpha agonist (assessed in the presence of epinephrine and excess beta antagonist), glucagon, and forskolin did not increase substantially with age and at 24 mo were less than the response to beta agonist. In hepatocyte homogenates adenylate cyclase activation by beta agonist but not glucagon and forskolin increased between 7 and 24 mo. These results suggest that adrenergic stimulation of glycogenolysis, which in young adult male rats is generally attributed to alpha adrenergic-mediated processes, becomes mediated predominantly by beta adrenergic-responsive adenylate cyclase during post-maturational aging.

AB - Adrenergic-stimulated glycogenolysis (estimated as glucose output) was determined in hepatocytes from 7, 14, 20, and 24 mo old male Fischer 344 rats. Glucose output in response to the beta adrenergic agonist isoproterenol was minimal at 7 mo but increased progressively with increasing age. At all ages the isoproterenol response was concentration dependent and was inhibited by the beta adrenergic antagonist propranolol. Stimulation of glucose output by the mixed alpha-beta agonist epinephrine also increased between 7 and 24 mo. Glycogenolytic responses to alpha agonist (assessed in the presence of epinephrine and excess beta antagonist), glucagon, and forskolin did not increase substantially with age and at 24 mo were less than the response to beta agonist. In hepatocyte homogenates adenylate cyclase activation by beta agonist but not glucagon and forskolin increased between 7 and 24 mo. These results suggest that adrenergic stimulation of glycogenolysis, which in young adult male rats is generally attributed to alpha adrenergic-mediated processes, becomes mediated predominantly by beta adrenergic-responsive adenylate cyclase during post-maturational aging.

UR - http://www.scopus.com/inward/record.url?scp=0023483251&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023483251&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(87)90990-9

DO - 10.1016/0006-291X(87)90990-9

M3 - Article

VL - 147

SP - 724

EP - 730

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -