ELXR: a resource for rapid exon-directed sequence analysis

Jeoffrey J. Schageman, Christopher J. Horton, Sijing Niu, Harold R. Garner, Alexander Pertsemlidis

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

ELXR (Exon Locator and Extractor for Resequencing) streamlines the process of determining exon/intron boundaries and designing PCR and sequencing primers for high-throughput resequencing of exons. We have pre-computed ELXR primer sets for all exons identified from the human, mouse, and rat mRNA reference sequence (RefSeq) public databases curated by the National Center for Biotechnology Information. The resulting exon-flanking PCR primer pairs have been compiled into a system called ELXRdb, which may be searched by keyword, gene name or RefSeq accession number.

Original languageEnglish (US)
Article numberR36
JournalGenome biology
Volume5
Issue number5
DOIs
StatePublished - May 2004
Externally publishedYes

Keywords

  • Design Polymerase Chain Reaction Primer
  • Genomic Contig
  • Genomic Extent
  • Polymerase Chain Reaction Prime Pair
  • Polymerase Chain Reaction Product Size

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Cell Biology

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