Elution of fibronectin from collagen with chaotrophic agents

Robert J. Klebe, Kevin L. Bentley, Penny J. Sasser, Robert C. Schoen

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Several compounds have been described which elute fibronectin from a gelatin-Sepharose affinity support. In the present study, it has been found that the potent chaotrophic agent, lithium di-iodosalicylic acid, is 20-fold more effective in eluting fibronectin from collagen than any other presently described fibronectin elution agent. Lithium di-iodosalicylic acid and certain other fibronectin elution agents have been characterized in regard to several parameters involved in the elution of fibronectin from collagen and plastic substrata. By assaying for retention of the cell adhesive activity of fibronectin, it has been demonstrated that 8 M urea + 0.1 M citric acid, pH 4.7, is the most effective condition for preservation of biological activity following elution of fibronectin from the gelatin-Sepharose affinity support.

Original languageEnglish (US)
Pages (from-to)111-117
Number of pages7
JournalExperimental Cell Research
Volume130
Issue number1
DOIs
StatePublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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