Elution of fibronectin from collagen with chaotrophic agents

Robert J. Klebe; Kevin L. Bentley; Penny J. Sasser; Robert C. Schoen

doi:10.1016/0014-4827(80)90047-6

Elution of fibronectin from collagen with chaotrophic agents

Robert J. Klebe, Kevin L. Bentley, Penny J. Sasser, Robert C. Schoen

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Several compounds have been described which elute fibronectin from a gelatin-Sepharose affinity support. In the present study, it has been found that the potent chaotrophic agent, lithium di-iodosalicylic acid, is 20-fold more effective in eluting fibronectin from collagen than any other presently described fibronectin elution agent. Lithium di-iodosalicylic acid and certain other fibronectin elution agents have been characterized in regard to several parameters involved in the elution of fibronectin from collagen and plastic substrata. By assaying for retention of the cell adhesive activity of fibronectin, it has been demonstrated that 8 M urea + 0.1 M citric acid, pH 4.7, is the most effective condition for preservation of biological activity following elution of fibronectin from the gelatin-Sepharose affinity support.

Original language	English (US)
Pages (from-to)	111-117
Number of pages	7
Journal	Experimental Cell Research
Volume	130
Issue number	1
DOIs	https://doi.org/10.1016/0014-4827(80)90047-6
State	Published - 1980
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

Access to Document

10.1016/0014-4827(80)90047-6

Cite this

@article{b765c600a27d4f3aad561acdf23d48f1,

title = "Elution of fibronectin from collagen with chaotrophic agents",

abstract = "Several compounds have been described which elute fibronectin from a gelatin-Sepharose affinity support. In the present study, it has been found that the potent chaotrophic agent, lithium di-iodosalicylic acid, is 20-fold more effective in eluting fibronectin from collagen than any other presently described fibronectin elution agent. Lithium di-iodosalicylic acid and certain other fibronectin elution agents have been characterized in regard to several parameters involved in the elution of fibronectin from collagen and plastic substrata. By assaying for retention of the cell adhesive activity of fibronectin, it has been demonstrated that 8 M urea + 0.1 M citric acid, pH 4.7, is the most effective condition for preservation of biological activity following elution of fibronectin from the gelatin-Sepharose affinity support.",

author = "Klebe, {Robert J.} and Bentley, {Kevin L.} and Sasser, {Penny J.} and Schoen, {Robert C.}",

note = "Funding Information: We wish to thank Mrs Bettye Moore for aid in preparation of the manuscript. This study was supported, in part, by grants CA 19017a nd GM 21433f rom the NIH.",

year = "1980",

doi = "10.1016/0014-4827(80)90047-6",

language = "English (US)",

volume = "130",

pages = "111--117",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Elution of fibronectin from collagen with chaotrophic agents

AU - Klebe, Robert J.

AU - Bentley, Kevin L.

AU - Sasser, Penny J.

AU - Schoen, Robert C.

N1 - Funding Information: We wish to thank Mrs Bettye Moore for aid in preparation of the manuscript. This study was supported, in part, by grants CA 19017a nd GM 21433f rom the NIH.

PY - 1980

Y1 - 1980

N2 - Several compounds have been described which elute fibronectin from a gelatin-Sepharose affinity support. In the present study, it has been found that the potent chaotrophic agent, lithium di-iodosalicylic acid, is 20-fold more effective in eluting fibronectin from collagen than any other presently described fibronectin elution agent. Lithium di-iodosalicylic acid and certain other fibronectin elution agents have been characterized in regard to several parameters involved in the elution of fibronectin from collagen and plastic substrata. By assaying for retention of the cell adhesive activity of fibronectin, it has been demonstrated that 8 M urea + 0.1 M citric acid, pH 4.7, is the most effective condition for preservation of biological activity following elution of fibronectin from the gelatin-Sepharose affinity support.

AB - Several compounds have been described which elute fibronectin from a gelatin-Sepharose affinity support. In the present study, it has been found that the potent chaotrophic agent, lithium di-iodosalicylic acid, is 20-fold more effective in eluting fibronectin from collagen than any other presently described fibronectin elution agent. Lithium di-iodosalicylic acid and certain other fibronectin elution agents have been characterized in regard to several parameters involved in the elution of fibronectin from collagen and plastic substrata. By assaying for retention of the cell adhesive activity of fibronectin, it has been demonstrated that 8 M urea + 0.1 M citric acid, pH 4.7, is the most effective condition for preservation of biological activity following elution of fibronectin from the gelatin-Sepharose affinity support.

UR - http://www.scopus.com/inward/record.url?scp=0019217766&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019217766&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(80)90047-6

DO - 10.1016/0014-4827(80)90047-6

M3 - Article

C2 - 7449840

AN - SCOPUS:0019217766

VL - 130

SP - 111

EP - 117

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -

Elution of fibronectin from collagen with chaotrophic agents

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this