Elevations of intracellular cAMP result in a change in cell shape that resembles dome formation in cultured rat glomerular epithelial cells

J. I. Kreisberg, P. Y. Patel, M. A. Venkatachalam, G. Taylor

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Cultured glomerular epithelial cells form a continuous monolayer of polyhedral-shaped cells. PGE2 (1 μg/ml) in the presence of the phosphodiesterase inhibitor isobutylmethylxanthine (MIX) markedly raises intracellular and medium cyclic AMP (cAMP) levels at 20 min (intracellular: MIX alone, 112 ± 6.6 pmol cAMP/mg protein, MIX plus PGE2, 2252±63 pmol cAMP/mg protein; medium: MIX, 20.6±2.1 pmol cAMP/mg protein; MIX plus PGE2, 117±3.8 pmol cAMP/mg protein). By 2 h, when cellular and medium cAMP levels were still elevated, the cells underwent a change in shape that was similar to dome formation (15 to 20% of the monolayer changing shape). Derivatives of cAMP [i. e. dibutyryl and 8-(4-chlorophenylthio)-cAMP], when added to the incubation medium also caused shape change in glomerular epithelial cells at 2 h; cAMP itself did not. The formation of domes has been used as a morphological indicator of the vertorial transport of salt and water in other cultured epithelial cells.

Original languageEnglish (US)
Pages (from-to)392-396
Number of pages5
JournalIn Vitro Cellular & Developmental Biology
Volume22
Issue number7
DOIs
Publication statusPublished - Jul 1 1986

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Keywords

  • cAMP
  • culture
  • dome formation
  • glomerular epithelial cells

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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