Elevated expression of monocyte chemoattractant protein 1 by vascular smooth muscle cells in hypercholesterolemic primates

X. Yu, S. Dluz, D. T. Graves, L. Zhang, H. N. Antoniades, W. Hollander, S. Prusty, A. J. Valente, C. J. Schwartz, G. E. Sonenshein

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Abstract

Atherosclerosis is marked by an overt inflammatory infiltrate, with enhanced recruitment of monocytes/macrophages observed in both human and experimental atherosclerosis. We previously determined that monocyte chemoattractant protein 1 (MCP-1) accounts for virtually all of the chemotactic activity produced by vascular (aortic) smooth muscle cells in culture. We now report that arteries from a primate model of atherosclerosis with dietary-induced hypercholesterolemia exhibit increased levels of MCP-1 mRNA expression in vivo, whereas their normal counterparts demonstrate minimal MCP-1 expression. Furthermore, immunohistochemistry and in situ hybridization clearly indicate that the expression of MCP-1 protein and mRNA is in the smooth muscle cells of the medial layer of the artery and in monocyte-like and smooth muscle-like cells found in the overlying intimal lesion. These studies indicate that one of the responses to dietary hypercholesterolemia is the expression of MCP-1 by vascular smooth muscle cells. This expression, when augmented with other cellular and molecular factors, could significantly contribute to the recruitment of monocytes/macrophages to the vessel wall.

Original languageEnglish (US)
Pages (from-to)6953-6957
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number15
DOIs
StatePublished - Aug 17 1992

Keywords

  • atherosclerosis
  • immunohistochemistry
  • in situ hybridization

ASJC Scopus subject areas

  • General

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    Yu, X., Dluz, S., Graves, D. T., Zhang, L., Antoniades, H. N., Hollander, W., Prusty, S., Valente, A. J., Schwartz, C. J., & Sonenshein, G. E. (1992). Elevated expression of monocyte chemoattractant protein 1 by vascular smooth muscle cells in hypercholesterolemic primates. Proceedings of the National Academy of Sciences of the United States of America, 89(15), 6953-6957. https://doi.org/10.1073/pnas.89.15.6953